Table 2 Epitopes identified in GP5 sequences from Costa Rica PRRSV-2 isolates Phylogenetic analysis of ORF5
Isolate from Costa Rica | Start | End | Match | Length of Match | Identity (%) | Epitope ID and Reference fron literature |
|---|---|---|---|---|---|---|
RA5_Puriscal-SanJose-CR_2019 | 29 | 37 | SQLQLIYNL | 9 | 88.9 | Gp5-SL,Ostrowski et al. (2002), PRRSV2 |
141 | 155 | KGKLYRWRSPVIIEK | 15 | 86.7 | Gp5-KK, Vashisht et al. (2008), PRRSV2, aa149–163 | |
179 | 192 | TPITKVSAEQWGRP | 14 | 85.7 | Gp5-TP, de Lima et al. (2006), PRRSV 2. aa 187–200 | |
44 | 53 | GTEWLAGKFD | 10 | 80.0 | Epitope C Guo et al. 2019, 52-61aa | |
C4_Corredores-Puntarenas-CR_2019 | 37 | 45 | SQLQLIYNL | 9 | 88.9 | Gp5-SL,Ostrowski et al. (2002), PRRSV2 |
117 | 131 | LAAFICFIIRAAKNC | 15 | 80.0 | Gp5-LC, Vashisht et al. (2008), PRRSV2, aa 117–131 | |
149 | 163 | KGRLYRWRSPVIIEK | 15 | 93.3 | Gp5-KK, Vashisht et al. (2008), PRRSV2, aa149–163 | |
187 | 200 | TPITKVSAEQWGRP | 14 | 85.7 | Gp5-TP, de Lima et al. (2006), PRRSV 2. aa 187–200 | |
52 | 61 | GTDWLAGKFD | 10 | 90.0 | Epitope C Guo et al. 2019, 52-61aa | |
D8_SanRamon-Alajuela-CR_2019 | 37 | 45 | SQLQLIYNL | 9 | 88.9 | Gp5-SL,Ostrowski et al. (2002), PRRSV2 |
149 | 163 | KGKLYRWRSPVIIEK | 15 | 86.7 | Gp5-KK, Vashisht et al. (2008), PRRSV2, aa149–163 | |
187 | 200 | TPITKVSAEQWGRP | 14 | 85.7 | Gp5-TP, de Lima et al. (2006), PRRSV 2. aa 187–200 | |
52 | 61 | GTDWLAGKFD | 10 | 90.0 | Epitope C Guo et al. 2019, 52-61aa | |
R2_SantaBarbara-Heredia-CR_2019 | 37 | 45 | SHLQLIYNL | 9 | 100.0 | Gp5-SL,Ostrowski et al. (2002), PRRSV2 |
117 | 131 | LAALTCFTIRFAKNC | 15 | 80.0 | Gp5-LC, Vashisht et al. (2008), PRRSV2, aa 117–131 | |
149 | 163 | KGRLYRWRSPVIIEK | 15 | 93.3 | Gp5-KK, Vashisht et al. (2008), PRRSV2, aa149–163 | |
187 | 200 | TPVTRVSAEQWGRP | 14 | 92.9 | Gp5-TP, de Lima et al. (2006), PRRSV 2. aa 187–200 | |
27 | 30 | VLVN | 4 | 100.0 | Epitope A, Guo et al. 2019, 27-30 aa | |
52 | 61 | GTDWLAGKFD | 10 | 90.0 | Epitope C Guo et al. 2019, 52-61aa | |
37 | 45 | SHLQLIYNL | 9 | 88.9 | Epitope B, Guo et al. 2019, 37-45aa | |
PA3_Coris-Cartago-CR_2019 liz | 22 | 36 | KGRLYRWRSPVIIEK | 15 | 93.3 | Gp5-KK, Vashisht et al. (2008), PRRSV2, aa149–163 |
D7_SanRamon-Alajuela-CR_2019 | 105 | 119 | KGKLYRWRSPVIIEK | 15 | 86.7 | Gp5-KK, Vashisht et al. (2008), PRRSV2, aa149–163 |
143 | 156 | TPITKVSAEQWGRP | 14 | 85.7 | Gp5-TP, de Lima et al. (2006), PRRSV 2. aa 187–200 | |
8 | 17 | GTDWLAGKFD | 10 | 90.0 | Epitope C Guo et al. 2019, 52-61aa | |
RA7_Puriscal-SanJose-CR_2019 | 37 | 45 | SQLQLIYNL | 9 | 88.9 | Gp5-SL,Ostrowski et al. (2002), PRRSV2 |
149 | 163 | KGKLYRWRSPVIIEK | 15 | 86.7 | Gp5-KK, Vashisht et al. (2008), PRRSV2, aa149–163 | |
52 | 61 | GTEWLAGKFD | 10 | 80.0 | Epitope C Guo et al. 2019, 52-61aa |