Table 4 Primers used for qRT-PCR
Gene | Accession number | Annealing temperature |  | Sequence (5′- > 3′) | Length | Tm |
|---|---|---|---|---|---|---|
VEGFA | NM_001316955.1 | 65 °C | F | AAAGTCTGGAGTGTGTGCCC | 20 | 60.18 |
R | GCTGGCTTTGGTGAGGTTTG | 20 | 59.97 | |||
TGFB2 | XM_010813095.3 | F | CAGAGTGCCTGAACAACGGA | 20 | 60.25 | |
R | TCCCAGGTTCCTGTCTTTATGG | 22 | 59.42 | |||
PRDM1 | NM_001192936.1 | F | CGGAGGCTTCCTTACCGAG | 19 | 59.56 | |
R | CCTCTGGAATAGATCCGCCAAAA | 23 | 60.43 | |||
SAMSN1 | NM_001035404.1 | F | TTCGACTGGGGTATGCGAAG | 20 | 59.83 | |
R | TGTTCCGGTATGGGAGGGTA | 20 | 59.66 | |||
BHLHE40 | NM_001024929.1 | F | AGGACAGCAAGGAGACCTACA | 21 | 60.2 | |
R | ACTGCTTTTTCCAAGTGACCC | 21 | 58.97 | |||
NR4A2 | NM_001076208.1 | F | TAAGTTTCCCTCGCCCCAAC | 20 | 59.96 | |
R | CTAAAGCCCCCTGTCTCGTC | 20 | 59.82 | |||
DUSP6 | NM_001046195.1 | F | TTAGGAGCCGCTGGACTTTT | 20 | 59.31 | |
R | CGCTCGTTCTCGGTGTCAAT | 20 | 60.73 | |||
EDN1 | NM_181010.2 | F | AAGAACTCAGGGACCAAGACTC | 22 | 59.36 | |
R | CTGTTGCTGATGGCCTCCAA | 20 | 60.61 | |||
MYL9 | NM_001075234.1 | F | GATGACTAGGCCATCCCAGC | 20 | 59.96 | |
R | TCTCAGCCCGTTTCCTTCAC | 20 | 59.97 | |||
PTGS2 | NM_174445.2 | F | ATCATTCACCAGGCAAAGGGC | 21 | 61.24 | |
R | TTCCTTCTCTCCTGTAAGTTCCTCA | 25 | 60.7 | |||
CXCL8 | NM_173925.2 | F | CTGGACAGCAGAGCTCACAA | 21 | 59.97 | |
R | CTGGACAGCAGAGCTCACAA | 20 | 60.04 | |||
IL1A | NM_174092.1 | F | GCTCGGTTCAGCAAAGAAGT | 20 | 58.77 | |
R | CGAAGTGGCTCATAGCTTGC | 20 | 59.35 | |||
β-actin | AF191490.1 | F | CAGAAGGACTCGTACGTGGG | 20 | 59.83 | |
R | CCGTGCTCAATGGGGTACTT | 20 | 60.04 |