Table 2 Statistics from mRNA-seq for peripheral lymphocytes in Jersey cattle under heat stress
Sample | Raw reads | Raw bases | Clean reads | Clean bases | Error rate (%) | Q20 (%) | Q30 (%) | GC (%) | Total reads | Total mapped reads | Reads mapped to multiple loci | Uniquely mapped reads |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
THI = 77_1 | 61,562,712 | 9,276,910,694 | 61,069,186 | 9.1 Gb | 0.0231 | 98.86 | 96.05 | 51.33 | 61,069,186 | 58,163,771 (95.24%) | 1,580,043 (2.59%) | 56,583,728 (92.66%) |
THI = 77_2 | 59,409,952 | 8,952,561,296 | 58,867,260 | 8.8 Gb | 0.0230 | 98.90 | 96.19 | 51.49 | 58,867,260 | 56,022,325 (95.17%) | 1,623,579 (2.76%) | 54,398,746 (92.41%) |
THI = 77_3 | 67,050,696 | 10,103,532,968 | 66,516,354 | 9.9 Gb | 0.0228 | 98.97 | 96.41 | 51.37 | 66,516,354 | 63,369,583 (95.27%) | 1,426,145 (2.14%) | 61,943,438 (93.13%) |
THI = 77_4 | 58,325,238 | 8,788,851,462 | 57,799,350 | 8.6 Gb | 0.0228 | 98.95 | 96.37 | 51.41 | 57,799,350 | 55,156,163 (95.43%) | 1,315,965 (2.28%) | 53,840,198 (93.15%) |
THI = 77_5 | 69,485,932 | 8,957,561,292 | 55,867,460 | 8.8 Gb | 0.0229 | 98.94 | 96.29 | 51.48 | 57,867,265 | 56,214,338 (95.17%) | 1,633,578 (2.76%) | 53,396,721 (92.41%) |
THI = 82_1 | 50,837,238 | 7,660,716,518 | 50,474,676 | 7.5 Gb | 0.0229 | 98.91 | 96.22 | 51.25 | 50,474,676 | 48,100,973 (95.3%) | 1,022,965 (2.03%) | 47,078,008 (93.27%) |
THI = 82_2 | 58,619,002 | 8,833,461,136 | 58,178,126 | 8.7 Gb | 0.0228 | 98.96 | 96.38 | 51.84 | 58,178,126 | 55,529,590 (95.45%) | 1,621,922 (2.79%) | 53,907,668 (92.66%) |
THI = 82_3 | 50,511,366 | 7,611,557,460 | 50,049,474 | 7.4 Gb | 0.0232 | 98.79 | 95.90 | 51.77 | 50,049,474 | 47,388,832 (94.68%) | 1,201,415 (2.4%) | 46,187,417 (92.28%) |
THI = 82_4 | 59,646,870 | 8,988,057,544 | 59,215,152 | 8.8 Gb | 0.0227 | 98.99 | 96.45 | 51.40 | 59,215,152 | 56,428,062 (95.29%) | 1,484,362 (2.51%) | 54,943,700 (92.79%) |
THI = 82_5 | 63,367,910 | 9,548,852,684 | 62,924,034 | 9.4 Gb | 0.0228 | 98.98 | 96.44 | 52.07 | 62,924,034 | 59,770,598 (94.99%) | 1,575,192 (2.5%) | 58,195,406 (92.49%) |
THI = 88_1 | 62,085,400 | 9,355,746,764 | 61,551,498 | 9.2 Gb | 0.0229 | 98.91 | 96.25 | 52.02 | 61,551,498 | 58,767,150 (95.48%) | 1,654,125 (2.69%) | 57,113,025 (92.79%) |
THI = 88_2 | 59,070,634 | 8,901,656,122 | 58,633,654 | 8.7 Gb | 0.0227 | 99.00 | 96.50 | 52.42 | 58,633,654 | 55,752,783 (95.09%) | 1,825,538 (3.11%) | 53,927,245 (91.97%) |
THI = 88_3 | 59,661,384 | 8,990,075,482 | 59,176,084 | 8.8 Gb | 0.0229 | 98.93 | 96.30 | 51.77 | 59,176,084 | 56,335,828 (95.2%) | 1,601,284 (2.71%) | 54,734,544 (92.49%) |
THI = 88_4 | 69,221,370 | 10,430,405,822 | 68,728,564 | 10.2 Gb | 0.0227 | 98.99 | 96.45 | 50.44 | 68,728,564 | 65,469,192 (95.26%) | 1,737,699 (2.53%) | 63,731,493 (92.73%) |
THI = 88_5 | 61,949,988 | 9,335,032,514 | 61,437,404 | 9.2 Gb | 0.0230 | 98.90 | 96.19 | 51.46 | 61,437,404 | 58,563,815 (95.32%) | 1,402,307 (2.28%) | 57,161,508 (93.04%) |