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Table 5 Bacterial enzyme activity[μmol/h/g digesta] in the colonic digesta of rabbits (mean ± SEM1)

From: The effect of dietary supplementation with silkworm pupae meal on gastrointestinal function, nitrogen retention and blood biochemical parameters in rabbits

 

Group2

p value

SBM

SPM5

SPM10

α-Glucosidase

 Extracellular

2.31 ± 0.273a

2.07 ± 0.182ab

1.50 ± 0.123b

0.018

 Intracellular

1.38 ± 0.259

1.40 ± 0.192

1.03 ± 0.100

0.241

 Total

3.70 ± 0.225a

3.47 ± 0.292a

2.53 ± 0.134b

0.003

 Release rate3

62.6 ± 6.190

60.3 ± 3.227

59.3 ± 3.604

0.658

α-Galactosidase

 Extracellular

3.89 ± 0.385a

2.56 ± 0.276b

1.73 ± 0.169b

< 0.001

 Intracellular

4.62 ± 0.478

4.16 ± 0.366

3.53 ± 0.466

0.135

 Total

8.51 ± 0.419a

6.70 ± 0.613b

5.26 ± 0.432b

< 0.001

 Release rate3

46.0 ± 4.475

37.9 ± 1.381

34.5 ± 3.874

0.051

β-Glucuronidase

 Extracellular

13.4 ± 1.263a

9.83 ± 1.159b

9.32 ± 0.797b

0.028

 Intracellular

38.4 ± 2.579

33.4 ± 2.343

36.2 ± 3.123

0.263

 Total

51.8 ± 2.972

43.2 ± 1.468

45.5 ± 3.131

0.054

 Release rate3

26.0 ± 2.106

23.4 ± 3.512

21.3 ± 2.294

0.293

β-Cellobiosidase

 Extracellular

0.235 ± 0.037b

0.383 ± 0.065ab

0.491 ± 0.074a

0.014

 Intracellular

0.357 ± 0.052

0.281 ± 0.041

0.349 ± 0.055

0.344

 Total

0.592 ± 0.056

0.663 ± 0.098

0.840 ± 0.120

0.117

 Release rate3

40.7 ± 5.621b

55.6 ± 4.220a

57.4 ± 4.332a

0.038

NAGase

 Extracellular

1.01 ± 0.073b

1.94 ± 0.253a

2.51 ± 0.302a

< 0.001

 Intracellular

1.98 ± 0.332

1.99 ± 0.162

2.05 ± 0.359

0.897

 Total

2.99 ± 0.372b

3.92 ± 0.391ab

4.55 ± 0.468a

0.027

 Release rate3

36.7 ± 3.457b

48.6 ± 2.239a

55.9 ± 4.314a

0.002

  1. a, bmeans with different superscripts in the same row differ significantly (P < 0.05)
  2. 1SEM - standard error of the mean
  3. 2Group: SBM - 10% SBM, SPM5–5% SBM and 5% SPM, SPM10–10% SPM (SBM - soybean meal, SPM - dried silkworm pupae meal)
  4. 3Release rate = extracellular enzyme activity expressed as a percentage of total (extra- + intracellular) enzyme activity