Molecular detection of Theileria species, Anaplasma species, Candidatus Mycoplasma haemobos, Trypanosoma evansi and first evidence of Theileria sinensis-associated bovine anaemia in crossbred Kedah-Kelantan x Brahman cattle

Agina, Onyinyechukwu Ada; Shaari, Mohd Rosly; Isa, Nur Mahiza Md; Ajat, Mokrish; Zamri-Saad, Mohd; Mazlan, Mazlina; Muhamad, Azim Salahuddin; Kassim, Afrah Alhana; Ha, Lee Chai; Rusli, Fairuz Hazwani; Masaud, Darulmuqaamah; Hamzah, Hazilawati

doi:10.1186/s12917-021-02902-0

Table 1 Genus and species-specific primers, thermocyclic conditions and amplicon size of the various genes used for the detection and amplification of cattle blood pathogens. Thermocyclic conditions include initial denaturing (ID), denaturing (D), annealing (A), extension (E), and final extension (FE)

From: Molecular detection of Theileria species, Anaplasma species, Candidatus Mycoplasma haemobos, Trypanosoma evansi and first evidence of Theileria sinensis-associated bovine anaemia in crossbred Kedah-Kelantan x Brahman cattle

Blood Pathogens	Gene	Primer Sequences (5′-3′) Forward (F) and Reverse (R)	Thermocyclic conditions	Base pair (bp)	References
Anaplasma species	16SrRNA	EHR16SD - GGTACCYACAGAAGAAGTCC EHR 16SR -TAGCACTCATCGTTTACAGC	ID: 95 °C /5mins D: 95 °C /30s A: 55 °C /30s E: 72 °C /90s No of cycles: 40 FE: 72 °C /5mins	345	[47]
Theileria species	18SrRNA	F-ACCTGGTTGATCCTGCCAG R-GATCCTTCGCAGGTTCACCTAC	ID: 94 °C /5mins D: 94 °C /30s A: 60 °C /30s E: 72 °C /1 min No of cycles: 40 FE: 72 °C /5mins	1750	[48]
Mycoplasma species	16SrRNA	F-GGGAGCAAACAGGATTAGATACCCT R-TGCACCATCTGTCACTCTGTTAACCTC	ID: 94 °C /5mins D: 94 °C /30s A: 55 °C /30s E: 72 °C /1 min50secs No of cycles: 40 FE: 72 °C/10 mins	280	[49]
Anaplasma marginale	MSP4	F- CATCTCCCATGAGTCACGAAGTGGC R-GCTGAACAGGAATCTTGCTCCAAG	ID: 95 °C /5mins D: 95 °C /1 min A: 65 °C /2mins E: 72 °C /1 min No of cycles: 40 FE:72 °C/10 min	761	[50]
Theileria orientalis	MPSP	F- CTTTGCCTAGGATACTTCCT R- ACGGCAAGTGGTGAGAACT	ID: 94 °C /4mins D: 94 °C /1 min A: 63 °C /1 min E: 72 °C /1 min No of cycles: 40 FE:72 °C /7mins	776	[51]
Theileria sinensis	MPSP	Tsin F- CACTGCTATGTTGTCCAAGAGATATT Tsin R-AATGCGCCTAAAGATAGTAGAAAAC	ID: 94 °C /3mins D: 94 °C /1 min A: 56 °C /1 min E: 72 °C /1 min No of cycles: 40 FE:72 °C /7mins	887	[52]
Trypanosoma evansi	RoTaT 1.2 VSG	F-GCG GGG TGT TTA AAG CAA TA R-ATT AGT GCT GCG TGT GTT CG	ID: 94 °C/4mins D: 94 °C /1 min A: 59 °C /1 min E: 72 °C /1 min No of cycles:40 FE: 72 °C/5mins	205	[53]
Candidatus Mycoplasma haemobos	16SrRNA	F-GAGTTAGTTATTAAAGCTTTAT R-ATTCATGAGGTACTATCAGTTG	ID: 94 °C /5mins D: 94 °C /30s A: 55 °C /30s E: 72 °C /30s No of cycles: 40 FE:72 °C /7mins	279	[49]

Back to article page

ISSN: 1746-6148

Contact us

General enquiries: journalsubmissions@springernature.com

BMC Veterinary Research

Contact us