Table 3 Primer sequences, product sizes, and PCR conditions in this study
| Target genes |
Primer sequences (5′ to 3′) | Product size (bp) | PCR conditions | References |
|---|---|---|---|---|
| Salmonella genus specific gene | ||||
| invA |
F: AAACGTTGAAAAACTGAGGA R: TCGTCATTCCATTACCTACC | 199 |
-Initial denaturation (95 °C for 10 min) − 30 cycles of: - Denaturation (94 °C for 60 s) - Annealing (62 °C for 90 s) - Extension (72 °C for 90 s) - Final extension (72 °C for 10 min) | [29] |
| S. Enteritidis serovar specific gene | ||||
| Sdf |
F: AAATGTGTTTTATCTGATGCAAGAGG R: GTTCGTTCTTCTGGTACTTACGATGAC | 299 | [30] | |
| Virulence genes | ||||
| pagC |
F: CGCCTTTTCCGTGGGGTATGC R: GAAGCCGTTTATTTTTGTAGAGGAGATGTT | 454 |
-Initial denaturation (95 °C for 10 min) − 35 cycles of: - Denaturation (94 °C for 40 s) - Annealing (62 °C for 30 s) - Extension (72 °C for 40 s) - Final extension (72 °C for 10 min) | [21] |
| cdtB |
F: ACAACTGTCGCATCTCGCCCCGTCATT R: CAATTTGCGTGGGTTCTGTAGGTGCGAGT | 268 | ||
| msgA |
F: GCCAGGCGCACGCGAAATCATCC R: GCGACCAGCCACATATCAGCCTCTTCAAAC | 189 | ||
| spaN |
F: AAAAGCCGTGGAATCCGTTAGTGAAGT R: CAGCGCTGGGGATTACCGTTTTG | 504 | ||
| tolC |
F: TACCCAGGCGCAAAAAGAGGCTATC R: CCGCGTTATCCAGGTTGTTGC | 161 | ||
| lpfC |
F: GCCCCGCCTGAAGCCTGTGTTGC R: AGGTCGCCGCTGTTTGAGGTTGGATA | 641 | ||
| Virulence plasmid-associated gene | ||||
| spvC |
F: ACTCCTTGCACAACCAAATGCGGA R: TCTCTTCTGCATTTCGCCACCATCA | 571 |
- Initial denaturation (94 °C for 5 min) −35 cycles of: - Denaturation (94 °C for 30 s) - Annealing (57 °C for 40 s) - Extension (72 °C for 30 s) - Final extension (72 °C for 10 min) | [31] |