Table 3 Primer sequences, product sizes, and PCR conditions in this study
Target genes | Primer sequences (5′ to 3′) | Product size (bp) | PCR conditions | References |
|---|---|---|---|---|
Salmonella genus specific gene | ||||
 invA | F: AAACGTTGAAAAACTGAGGA R: TCGTCATTCCATTACCTACC | 199 | -Initial denaturation (95 °C for 10 min) − 30 cycles of: - Denaturation (94 °C for 60 s) - Annealing (62 °C for 90 s) - Extension (72 °C for 90 s) - Final extension (72 °C for 10 min) | [29] |
S. Enteritidis serovar specific gene | Â | |||
 Sdf | F: AAATGTGTTTTATCTGATGCAAGAGG R: GTTCGTTCTTCTGGTACTTACGATGAC | 299 | [30] | |
Virulence genes | ||||
 pagC | F: CGCCTTTTCCGTGGGGTATGC R: GAAGCCGTTTATTTTTGTAGAGGAGATGTT | 454 | -Initial denaturation (95 °C for 10 min) − 35 cycles of: - Denaturation (94 °C for 40 s) - Annealing (62 °C for 30 s) - Extension (72 °C for 40 s) - Final extension (72 °C for 10 min) | [21] |
 cdtB | F: ACAACTGTCGCATCTCGCCCCGTCATT R: CAATTTGCGTGGGTTCTGTAGGTGCGAGT | 268 | ||
 msgA | F: GCCAGGCGCACGCGAAATCATCC R: GCGACCAGCCACATATCAGCCTCTTCAAAC | 189 | ||
 spaN | F: AAAAGCCGTGGAATCCGTTAGTGAAGT R: CAGCGCTGGGGATTACCGTTTTG | 504 | ||
 tolC | F: TACCCAGGCGCAAAAAGAGGCTATC R: CCGCGTTATCCAGGTTGTTGC | 161 | ||
 lpfC | F: GCCCCGCCTGAAGCCTGTGTTGC R: AGGTCGCCGCTGTTTGAGGTTGGATA | 641 | ||
Virulence plasmid-associated gene | ||||
 spvC | F: ACTCCTTGCACAACCAAATGCGGA R: TCTCTTCTGCATTTCGCCACCATCA | 571 | - Initial denaturation (94 °C for 5 min) −35 cycles of: - Denaturation (94 °C for 30 s) - Annealing (57 °C for 40 s) - Extension (72 °C for 30 s) - Final extension (72 °C for 10 min) | [31] |