The stimulation and inhibition of beta-2 adrenergic receptor on the inflammatory responses of ovary and immune system in the aged laying hens

Table 3 Chicken primers used for real-time PCR

Gene	Accession No.	primers sequences (5′ → 3′)	Orientation
β2ADR ¹	XM_004950587	GACGCCGGAACGCTGAG	Forward
β2ADR ¹	XM_004950587	GAAGACAGTGACCAGCACGA	Reverse
COX-1 ²	XM_425326	TCAGGTGGTTCTGGGACATCA	Forward
COX-1 ²	XM_425326	TGTAGCCGTACTGGGAGTTGAA	Reverse
COX-2 ³	XM_422297	CTGCTCCCTCCCATGTCAGA	Forward
COX-2 ³	XM_422297	CACGTGAAGAATTCCGGTGTT	Reverse
IL-1β ⁴	AB559570	CTTCCTCCAGCCAGAAAGT	Forward
IL-1β ⁴	AB559570	CAGCTTGTAGCCCTTGAT	Reverse
IL-6 ⁵	AB559572	CAACCTCAACCTGCCCAA	Forward
IL-6 ⁵	AB559572	GGAGAGCTTCCTCAGGCATT	Reverse
IL-10 ⁶	AB559574	CACAACTTCTTCACCTGCGAG	Forward
IL-10 ⁶	AB559574	CATGGCTTTGTAGATCCCGTTC	Reverse
TNF-α ⁷	AY765397	TGTGTATGTGCAGCAACCCGTAGT	Forward
TNF-α ⁷	AY765397	GGCATTGCAATTTGGACAGAAGT	Reverse
β-Actin ⁸	L08165	CATCACCATTGGCAATGAGAGG	Forward
β-Actin ⁸	L08165	GCAAGCAGGAGTACGATGAATC	Reverse

1) Beta-2 adrenergic receptor, 2) Cyclooxygenases-1, 3) Cyclooxygenases-2, 4) Interleukin-1β, 5) Interleukin − 6, 6) Interleukin − 10, and 7) Tumor necrosis factor-α. 8) β2ADR, COX-1, COX-2, IL-1β, IL-6, IL-10, and TNF-α mRNA data that are normalized by β-actin

ISSN: 1746-6148