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Fig. 1 | BMC Veterinary Research

Fig. 1

From: Identification of four insertion sites for foreign genes in a pseudorabies virus vector

Fig. 1

Construction of PRV-S(UL11-10)ΔTK/gE, PRV-S(UL35-36)ΔTK/gE and PRV-S(UL46-27)ΔTK/gE. a The S expression cassette with a kanamycin resistance gene was inserted into the noncoding area (UL11-10, UL35-36, UL46-27 or US2-1) through the first recombination to generate four recombinant BAC clones (BACPRV−S−KAN(UL11−10)ΔTK/gE/gI, BACPRV−S−KAN(UL35–36)ΔTK/gE/gI, BACPRV−S−KAN(UL46−27)ΔTK/gE/gI and BACPRV−S−KAN(US2−1)ΔTK/gE/gI). b The second recombination was performed to delete the kanamycin resistance gene and generate the final recombinants (BACPRV−S(UL11−10)ΔTK/gE/gI, BACPRV−S(UL35–36)ΔTK/gE/gI, BACPRV−S(UL46−27)ΔTK/gE/gI and BACPRV−S(US2−1)ΔTK/gE/gI). c Homologous recombination was performed to recover the intact gI gene and part of gE gene (1299 to 1735 bp of gE open reading frame). d Schematic presentation of the PRV-S(UL11-10)ΔTK/gE, PRV-S(UL35-36)ΔTK/gE and PRV-S(UL46-27)ΔTK/gE were shown

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