Table 1 Characteristics of bacterial strains, plasmids, and primers used in this study
From: The construction of recombinant Lactobacillus casei vaccine of PEDV and its immune responses in mice
Strain/plasmid/primer | Characteristics and/or sequences | Source/reference |
|---|---|---|
Strain | ||
DH5α | Genotype: supE44 ΔlacU169 (φ80lacZ△M15) hsdR17 recA1 endA1 gyrA96 thi-1 relA1 | TaKaRa (Otsu, Japan) |
BL21 | Genotype: F-ompT hsdS (rB-mB) gal dcm (DE3) | TaKaRa (Otsu, Japan) |
Lactobacillus casei | ATCC393, used as a vector system for targeted delivery of antigens in oral immunization. | Preserved in our lab |
Brucella abortus | Brucella abortus S19, supply gene sequence of omp16 gene | Preserved in our lab |
PEDV | AJ1102, variant strain, isolated in China. | Preserved in our lab |
Plasmids | ||
pET28a (+) | The expression vector of Escherichia coli, Kanr | Preserved in our lab |
pVE5523 | The expression vector of Lactobacillus casei, Ampr | BioVector NTCC |
pET28a-PEDVS | The recombinant vector of pET28a and S protein of PEDV, Kanr | This study |
pET28a-OMP16-PEDVS | The recombinant vector of pET28a and fusion protein (S protein of PEDV and OMP16 protein of Brucella abortus), Kanr | This study |
pVE5523-OMP16-PEDVS | The recombinant vector of pVE5523 and fusion protein (S protein of PEDV and OMP16 protein of Brucella abortus), Ampr | This study |
Primers | ||
PEDVS-F1/R1 | F1: CCGGAATTCATGCTGAGTCATGAACAGCC R1: TGCTCTAGATTAATATGCAGCCTGCTCTG | This study |
PEDVS-F2/R2 | F2:GGTGGTGGCGGTAGCGGCGGTGGTGGCTCTGGTGGCGGCGGTTCTTTCTTTTGTTACTTTGCCAT R2: CGCGATATCTTAATATGCAGCCTGCTCTG | This study |
OMP16-F/R | F: CGCGTCGACATGGCGTCAAAGAAGAACCTTCCG R:AGAACCGCCGCCACCAGAGCCACCACCGCCGCTACCGCCACCACCGGTACCCCGTCCGGCCCCGT | This study |