Table 3 The primers used for PCR for resistance genes in S. aureus isolates
Primer name | Sequence (5’-3’) | Annealing temperature(℃) | Size (bp) | References |
|---|---|---|---|---|
blaZ -F | AACACCTGCTGCTTTCGGTA | 55.5 | 314 | This study |
blaZ- R | CACTCTTGGCGGTTTCACTT | |||
mecA-F | CTTTGCTAGAGTAGCACTCG | 55.5 | 533 | Herold B.C., et al. |
mecA-R | GCTAGCCATTCCTTTATCTTG | |||
ermA-F | CTACACTTGGCTTAGGATGA | 56.5 | 311 | This study |
ermA-R | AGTGACTAAAGAAGCGGTAA | |||
ermB-F | TAACGACGAAACTGGCTAA | 56.0 | 414 | This study |
ermB-R | CTGTGGTATGGCGGGTAA | |||
ermC-F | GAGGCTCATAGACGAAGAAA | 54.5 | 375 | This study |
ermC-R | AAGTTCCCAAATTCGAGTAA | |||
aacA-aphD-F | ATTGAAGATTTGCCAGAACA | 56.5 | 178 | This study |
aacA-aphD-R | CACTATCATAACCACTACCG |