Table 2 Porcine specific primer sequences used to assess fetal apoptosis and hypoxia
Pathway | Target | NCBI Gene ID | Forward primer | Reverse primer | Tm (°C) | Length (bp) |
|---|---|---|---|---|---|---|
Apoptosis | CASP3 | 397,244 | 5′-GTGGGATTGAGACGGACAGT-3′ | 5′-TTCGCCAGGAATAGTAACCAG-3’ | 60 | 114 |
CASP7 | 100,156,777 | 5′-TCGGTGCAAGACCCTTTTAG-3’ | 5′-GCCTGGAACTGTGGAATAGG-3’ | 60 | 178 | |
CASP8 | 595,105 | 5′-GGAACTGCTTTTCCGAATGA-3’ | 5′-AGCATGACCCTGTAGGCAGA-3’ | 60 | 126 | |
CASP9 | 100,518,913 | 5′-TCTGCCCACACCTAGTGACA-3’ | 5′-ACAGCATTGGAGACCCTGAG-3’ | 60 | 171 | |
RIPK1 | 100,524,751 | 5′-ATCCTGTACGGCAACTCTGG-3’ | 5′-GGTGGTGTTCTCGAAGATGG-3’ | 60 | 147 | |
RIPK3 | 100,153,263 | 5′-AATAGGCCCTCCTTCCAAGA-3’ | 5′-CTCACGGACAGACAACAAGC-3’ | 60 | 162 | |
Infection | IDO1 | 100,519,877 | 5′-GCTGTCAGAGGGTCTGCTCT-3’ | 5′-TGAAGGAACTCCACCCACAG-3’ | 60 | 139 |
Hypoxia | HIF1α | 396,696 | 5′-GGCAGCAATGACACAGAAAC-3’ | 5′-CTGATTGAGTGCAGGGTCAG-3’ | 59 | 180 |
VEGFα | 397,157 | 5′-CAACATCGCCATGCAGATTA-3’ | 5′-GCATTCACATTTGTTGTGCTG-3’ | 60 | 91 | |
NOX1 | 100,739,822 | 5′-TCCCTTTACCCTGACCTCTG-3’ | 5′-TCCACCTCAATCCTTGGAAC-3’ | 60 | 132 | |
NOS2 | 396,859 | 5′-CTGTGAGACGTTCGATGTCC-3’ | 5′-GCTGCTGAGAGCTTTGTTGA-3’ | 59 | 139 | |
LDHA | 407,245 | 5′-TTCACCCCCTAAGCTGTCAT-3’ | 5′-TAAGCACTGTCCACCACCTG-3’ | 60 | 178 |