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Table 2 Porcine specific primer sequences used to assess fetal apoptosis and hypoxia

From: Fetal hypoxia and apoptosis following maternal porcine reproductive and respiratory syndrome virus (PRRSV) infection

Pathway Target NCBI Gene ID Forward primer Reverse primer Tm (°C) Length (bp)
Apoptosis CASP3 397,244 5′-GTGGGATTGAGACGGACAGT-3′ 5′-TTCGCCAGGAATAGTAACCAG-3’ 60 114
CASP7 100,156,777 5′-TCGGTGCAAGACCCTTTTAG-3’ 5′-GCCTGGAACTGTGGAATAGG-3’ 60 178
CASP8 595,105 5′-GGAACTGCTTTTCCGAATGA-3’ 5′-AGCATGACCCTGTAGGCAGA-3’ 60 126
CASP9 100,518,913 5′-TCTGCCCACACCTAGTGACA-3’ 5′-ACAGCATTGGAGACCCTGAG-3’ 60 171
RIPK1 100,524,751 5′-ATCCTGTACGGCAACTCTGG-3’ 5′-GGTGGTGTTCTCGAAGATGG-3’ 60 147
RIPK3 100,153,263 5′-AATAGGCCCTCCTTCCAAGA-3’ 5′-CTCACGGACAGACAACAAGC-3’ 60 162
Infection IDO1 100,519,877 5′-GCTGTCAGAGGGTCTGCTCT-3’ 5′-TGAAGGAACTCCACCCACAG-3’ 60 139
Hypoxia HIF1α 396,696 5′-GGCAGCAATGACACAGAAAC-3’ 5′-CTGATTGAGTGCAGGGTCAG-3’ 59 180
VEGFα 397,157 5′-CAACATCGCCATGCAGATTA-3’ 5′-GCATTCACATTTGTTGTGCTG-3’ 60 91
NOX1 100,739,822 5′-TCCCTTTACCCTGACCTCTG-3’ 5′-TCCACCTCAATCCTTGGAAC-3’ 60 132
NOS2 396,859 5′-CTGTGAGACGTTCGATGTCC-3’ 5′-GCTGCTGAGAGCTTTGTTGA-3’ 59 139
LDHA 407,245 5′-TTCACCCCCTAAGCTGTCAT-3’ 5′-TAAGCACTGTCCACCACCTG-3’ 60 178