Development of recombinase polymerase amplification assays for rapid and visual detection of canine distemper virus infecting giant panda

Table 4 Primers and probe information

Assay	Primers name	Position	Target gene	Sequence (5′ – 3′)
RT-RPA-VF	CDV-F	1310–1339	N	Biotin-AACGGAGGACCGGACGATTCGCACTGCTGG
	CDV-R	1438–1467		CATCATTGAAGTGGATGGGGTATTTGTCTC
	CDV-P	1374–1420		FITC-CTCTTGTTGATGGTTGGGGGTTGTTGA TTAG[THF]GACTTCGGATCTTTC-C3 Spacer
RT-PCR	GBJD-F	7823–7842	H	CGAGTCTTTGAGATAGGGTT
RT-PCR	GBJD-R	8258–8277	H	CCTCCAAAGGGTTCCCATGA
RT-qPCR	N-F	905–931		AGCTAGTTTCATCTTAACTATCAAATT
	N-R	965–987	N	TTAAATCCCCAGAAAACTCATGC
	N-P	934–963	N	FAM-ACCCGAGAGCCGGATACATAGTTTCAATGC-BHQ

All of primers and probe positions were based on the giant panda/SX/2014 strain of CDV (GenBank accession no. KP793921.1)

ISSN: 1746-6148