Table 2 Reaction time for RT-RPA-VF assays targeting the CDV N gene
Time | No. positive/ no. replications | ||||||||
|---|---|---|---|---|---|---|---|---|---|
Synthesized RNA transcript dilution (5 × copies/μl) | |||||||||
107 | 106 | 105 | 104 | 103 | 102 | 101 | 100 | N | |
15 min | 3/3 | 3/3 | 3/3 | 3/3 | 0/3 | 0/3 | 0/3 | 0/3 | 0/3 |
20 min | 3/3 | 3/3 | 3/3 | 3/3 | 3/3 | 3/3 | 0/3 | 0/3 | 0/3 |
25 min | 3/3 | 3/3 | 3/3 | 3/3 | 3/3 | 3/3 | 1/3 | 0/3 | 0/3 |
30 min | 3/3 | 3/3 | 3/3 | 3/3 | 3/3 | 3/3 | 3/3 | 0/3 | 0/3 |
35 min | 3/3 | 3/3 | 3/3 | 3/3 | 3/3 | 3/3 | 3/3 | 0/3 | 0/3 |