Fig. 3

Profiles of genes involved in iron homeostasis, antimicrobial responses and immune signaling in SHK-1 infected with P. salmonis and exposed to DFO. Gene profiles were determined by using SYBR green RT-qPCR in SHK-1 cells under four conditions (SHK-1, SHK-1 + DFO, SHK-1 + P. sal and SHK-1 + P. sal + DFO) at 4- and 7-days post infection (dpi). Each test gene was normalized to ELF-1α. Relative expression is expressed as fold-change over the control condition (SHK-1, non-infected SHK-1 cells without DFO) for each time point. Data represent the mean ± SD of three independent experiments. The asterisks represent significant differences compared to control or between experimental conditions (indicated with n-line); *ρ < 0.05, **ρ < 0.01, ***ρ < 0.001, ****ρ < 0.0001