Fig. 1

The evaluation of cytopathic effects in SHK-1 cells infected with P. salmonis. SHK-1 cells under four experimental conditions were evaluated microscopically for the cytopathic effects over 11 days, at 4, 7 and 11 days post infection (dpi). SHK-1 cells infected with P. salmonis were treated with DFO (SHK-1 + P. sal + DFO) and without it (SHK-1 + P. sal). Non-infected SHK-1 cells were treated with DFO (SHK-1 + DFO) to serve as a toxicity control. Positive (SHK-1 + P. sal) and negative controls (SHK-1) were used as references for treatment comparisons. For treatments with DFO, cells were pretreated with the chelator 24 h before infection. Images were taken with the EVOS® FL Color Imaging System (Invitrogen, Thermo Fisher Scientific Inc., Carlsbad, CA, USA) using 10X objective after 3 PBS-1X washes. The scale bar was added by using the image analysis ImageJ 1.37 software (National Institutes of Health, Bethesda, MD, USA)