Blood cultures and blood microbiota analysis as surrogates for bronchoalveolar lavage fluid analysis in dogs with bacterial pneumonia

Table 1 Match of bacterial taxa between BALF and blood (culture results versus most abundant bacteria from microbiome analysis)

Dog#	BALF Culture Result		Blood Culture Result	Most abundant genus (% RA)
Dog#	Genus	Species	Blood Culture Result	BALF		Blood		OP
1^a	Haemophilus^b	haemoglobinophilus	No growth	Haemophilus^b	(23.4)	Mycoplasma	(37.8)	Haemophilus	(26.2)
2^a	Pasteurella	canis	No growth	Acinetobacter	(19.4)	Streptococcus sp.	(18.5)	Frederiksenia^c	(31.7)
3	Pseudomonas	putida	No growth	Brevundimonas	(32.7)	Gemmata	(12.9)	Haemophilus	(68.0)
	Stenotrophomonas	maltophila
	Achromobacter	xylosoxidans
4	Pseudomonas	putida	No growth	Carnobacterium	(69.9)	Streptococcus	(23.6)	N/A
4	Pseudomonas	oleovorans	No growth	Carnobacterium	(69.9)	Streptococcus	(23.6)	N/A
5	Lactobacillus	salivarius	No growth	Lactobacillus	(56.2)	Cutibacterium	(30.7)	Lactobacillus	(21.7)
6	Escherichia^b	coli	No growth	Escherichia^b	(97.5)	Streptococcus	(13.4)	Haemophilus	(17.1)
7	Escherichia^b	coli	E. coli^b	Escherichia^b	(91.6)	Streptococcus^b	(15.4)	N/A
	Klebsiella^b	pneumoniae	Klebsiella pneumoniae^b
	Streptococcus^b	canis	Streptococcus canis^b
8	Klebsiella	oxytoca	Staphylococcus pseudintermedius	Acinetobacter^b	(55.8)	Acinetobacter^b	(83.9)	Alloprevotella	(20.7)
8	Streptococcus	canis	Staphylococcus pseudintermedius	Acinetobacter^b	(55.8)	Acinetobacter^b	(83.9)	Alloprevotella	(20.7)
9	Neisseria^b	weaveri	Neisseria weaveri^c	Frederiksenia^c	(37.1)	Acinetobacter	(61.6)	Frederiksenia^c	(24.3)
9	Ursidibacter^d	maritimus	Federiksenia canicola	Frederiksenia^c	(37.1)	Acinetobacter	(61.6)	Frederiksenia^c	(24.3)

BALF bronchoalveolar lavage fluid, OTU operational taxonomic unit, RA relative abundance, N/A not available (OP not collected)
^a Community-acquired pneumonia; ^b Represent identification of the same taxa via culture and 16S rRNA sequencing; ^c Frederiksenia sp. at the genus level comprised by a single OTU: Pasteurellaceae bacterium Orientalotternb1; ^d A Pasteurella like organism with no known pathogenic potential

ISSN: 1746-6148