Development of TaqMan-based real-time RT-PCR assay based on N gene for the quantitative detection of feline morbillivirus

Table 7 PCR protocol applied for two different primer sets of N gene

Step	Temperature	Time	Cycle
Initial denaturation	95 °C	1 min	1x
Denaturation	95 °C	15 s
Annealing	58 °C	1 min	35x
Extension	72 °C	1 min
Final extension	72 °C	5 min	1x
Hold	12 °C	∞	1x

ISSN: 1746-6148