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Table 7 PCR protocol applied for two different primer sets of N gene

From: Development of TaqMan-based real-time RT-PCR assay based on N gene for the quantitative detection of feline morbillivirus

Step Temperature Time Cycle
Initial denaturation 95 °C 1 min 1x
Denaturation 95 °C 15 s  
Annealing 58 °C 1 min 35x
Extension 72 °C 1 min  
Final extension 72 °C 5 min 1x
Hold 12 °C 1x