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Table 3 Detection of FeMV using conventional RT-PCR and qRT-PCR quantification from clinical samples

From: Development of TaqMan-based real-time RT-PCR assay based on N gene for the quantitative detection of feline morbillivirus

Sample Sample ID RT-PCR
(N gene)
Log copy number (mean ± S.D.) Source of cat Health status
Urine UPM23 + 6.60 ± 0.12 Client-owned Healthy
Urine UPM52 + 4.94 ± 0.02 Client-owned Healthy
Urine UPM53 + 4.93 ± 0.02 Client-owned Healthy
Urine UPM202 + 4.77 ± 0.02 Client-owned Healthy
Urine UPM203 BLD Client-owned Healthy
Urine UPM204 4.86 ± 0.06 Client-owned Healthy
Urine UPM205 4.55 ± 0.03 Client-owned Healthy
Urine UPM207 4.67 ± 0.02 Client-owned Healthy
Urine UPM210 + 3.66 ± 0.08 Client-owned Healthy
Urine UPM219 3.80 ± 0.14 Client-owned CKD stage IV
Urine UPM220 + 4.11 ± 0.05 Client-owned N/A
Urine UPM221 3.66 ± 0.02 Client-owned Healthy
Urine UPM224 BLD Client-owned Healthy
Urine UPM225 3.54 ± 0.01 Client-owned Healthy
Urine UPM226 3.71 ± 0.11 Client-owned Healthy
Urine UPM227 BLD Client-owned N/A
Urine UPM228 BLD Client-owned N/A
Urine UPM229 BLD Client-owned N/A
Urine UPM231 + 4.29 ± 0.23 Client-owned Healthy
Urine UPM232 BLD Client-owned CKD Stage II
Urine UPM240 BLD Client-owned Healthy
Urine UPM241 BLD Client-owned Healthy
Urine UPM271 BLD Client-owned Heart disease
Urine UPM272 BLD Client-owned CKD Stage II
Urine UPM273 BLD Client-owned N/A
Urine UPM274 BLD Client-owned Suspected CKD
Urine UPM275 BLD Client-owned FURD
Urine UPM276 + 4.05 ± 0.08 Client-owned Healthy
Urine UPM277 BLD Client-owned Healthy
Urine UPM278 BLD Client-owned N/A
Urine UPM279 BLD Client-owned N/A
Urine UPM280 BLD Client-owned Suspected CKD
Urine UPM281 BLD Client-owned N/A
Urine UPM282 4.63 ± 0.05 Client-owned FLUTD
Urine UPM283 BLD Client-owned Healthy
Urine UPM284 BLD Client-owned Healthy
Urine UPM285 3.74 ± 0.02 Client-owned N/A
Urine UPM286 3.88 ± 0.18 Client-owned Obstructive FLUTD
Urine UPM287 BLD Client-owned Suspected CKD
Urine UPM304 BLD Shelter N/A
Urine UPM305 + 4.34 ± 0.10 Shelter Platynosomum sp.
Urine UPM306 3.76 ± 0.26 Shelter N/A
Urine UPM307 BLD Shelter N/A
Urine UPM308 BLD Shelter Platynosomum sp.
Urine UPM310 BLD Shelter N/A
Urine UPM311 BLD Shelter Platynosomum sp.
Urine UPM312 BLD Shelter Platynosomum sp.
Urine UPM313 BLD Shelter N/A
Urine UPM314 + 4.45 ± 0.07 Shelter Platynosomum sp.
Urine UPM315 + 4.92 ± 0.07 Shelter Platynosomum sp.
Urine UPM316 BLD Shelter Platynosomum sp.
Urine UPM317 BLD Shelter N/A
Urine UPM318 BLD Shelter N/A
Urine UPM320 BLD Shelter N/A
Urine UPM321 BLD Shelter N/A
Kidney UPM304 3.80 ± 0.03 Shelter N/A
Kidney UPM305 BLD Shelter Platynosomum sp.
Kidney UPM306 BLD Shelter N/A
Kidney UPM307 BLD Shelter N/A
Kidney UPM308 BLD Shelter Platynosomum sp.
Kidney UPM310 BLD Shelter N/A
Kidney UPM311 BLD Shelter Platynosomum sp.
Kidney UPM312 3.70 ± 0.10 Shelter Platynosomum sp.
Kidney UPM313 BLD Shelter N/A
Kidney UPM314 BLD Shelter Platynosomum sp.
Kidney UPM315 BLD Shelter Platynosomum sp.
Kidney UPM316 BLD Shelter Platynosomum sp.
Kidney UPM317 BLD Shelter N/A
Kidney UPM318 BLD Shelter N/A
Kidney UPM320 BLD Shelter N/A
Kidney UPM321 3.89 ± 0.08 Shelter N/A
  1. BLD denotes below limit of detection of qRT-PCR; and
  2. N/A denotes not available