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Table 3 Detection of FeMV using conventional RT-PCR and qRT-PCR quantification from clinical samples

From: Development of TaqMan-based real-time RT-PCR assay based on N gene for the quantitative detection of feline morbillivirus

Sample

Sample ID

RT-PCR

(N gene)

Log copy number (mean ± S.D.)

Source of cat

Health status

Urine

UPM23

+

6.60 ± 0.12

Client-owned

Healthy

Urine

UPM52

+

4.94 ± 0.02

Client-owned

Healthy

Urine

UPM53

+

4.93 ± 0.02

Client-owned

Healthy

Urine

UPM202

+

4.77 ± 0.02

Client-owned

Healthy

Urine

UPM203

BLD

Client-owned

Healthy

Urine

UPM204

4.86 ± 0.06

Client-owned

Healthy

Urine

UPM205

4.55 ± 0.03

Client-owned

Healthy

Urine

UPM207

4.67 ± 0.02

Client-owned

Healthy

Urine

UPM210

+

3.66 ± 0.08

Client-owned

Healthy

Urine

UPM219

3.80 ± 0.14

Client-owned

CKD stage IV

Urine

UPM220

+

4.11 ± 0.05

Client-owned

N/A

Urine

UPM221

3.66 ± 0.02

Client-owned

Healthy

Urine

UPM224

BLD

Client-owned

Healthy

Urine

UPM225

3.54 ± 0.01

Client-owned

Healthy

Urine

UPM226

3.71 ± 0.11

Client-owned

Healthy

Urine

UPM227

BLD

Client-owned

N/A

Urine

UPM228

BLD

Client-owned

N/A

Urine

UPM229

BLD

Client-owned

N/A

Urine

UPM231

+

4.29 ± 0.23

Client-owned

Healthy

Urine

UPM232

BLD

Client-owned

CKD Stage II

Urine

UPM240

BLD

Client-owned

Healthy

Urine

UPM241

BLD

Client-owned

Healthy

Urine

UPM271

BLD

Client-owned

Heart disease

Urine

UPM272

BLD

Client-owned

CKD Stage II

Urine

UPM273

BLD

Client-owned

N/A

Urine

UPM274

BLD

Client-owned

Suspected CKD

Urine

UPM275

BLD

Client-owned

FURD

Urine

UPM276

+

4.05 ± 0.08

Client-owned

Healthy

Urine

UPM277

BLD

Client-owned

Healthy

Urine

UPM278

BLD

Client-owned

N/A

Urine

UPM279

BLD

Client-owned

N/A

Urine

UPM280

BLD

Client-owned

Suspected CKD

Urine

UPM281

BLD

Client-owned

N/A

Urine

UPM282

4.63 ± 0.05

Client-owned

FLUTD

Urine

UPM283

BLD

Client-owned

Healthy

Urine

UPM284

BLD

Client-owned

Healthy

Urine

UPM285

3.74 ± 0.02

Client-owned

N/A

Urine

UPM286

3.88 ± 0.18

Client-owned

Obstructive FLUTD

Urine

UPM287

BLD

Client-owned

Suspected CKD

Urine

UPM304

BLD

Shelter

N/A

Urine

UPM305

+

4.34 ± 0.10

Shelter

Platynosomum sp.

Urine

UPM306

3.76 ± 0.26

Shelter

N/A

Urine

UPM307

BLD

Shelter

N/A

Urine

UPM308

BLD

Shelter

Platynosomum sp.

Urine

UPM310

BLD

Shelter

N/A

Urine

UPM311

BLD

Shelter

Platynosomum sp.

Urine

UPM312

BLD

Shelter

Platynosomum sp.

Urine

UPM313

BLD

Shelter

N/A

Urine

UPM314

+

4.45 ± 0.07

Shelter

Platynosomum sp.

Urine

UPM315

+

4.92 ± 0.07

Shelter

Platynosomum sp.

Urine

UPM316

BLD

Shelter

Platynosomum sp.

Urine

UPM317

BLD

Shelter

N/A

Urine

UPM318

BLD

Shelter

N/A

Urine

UPM320

BLD

Shelter

N/A

Urine

UPM321

BLD

Shelter

N/A

Kidney

UPM304

3.80 ± 0.03

Shelter

N/A

Kidney

UPM305

BLD

Shelter

Platynosomum sp.

Kidney

UPM306

BLD

Shelter

N/A

Kidney

UPM307

BLD

Shelter

N/A

Kidney

UPM308

BLD

Shelter

Platynosomum sp.

Kidney

UPM310

BLD

Shelter

N/A

Kidney

UPM311

BLD

Shelter

Platynosomum sp.

Kidney

UPM312

3.70 ± 0.10

Shelter

Platynosomum sp.

Kidney

UPM313

BLD

Shelter

N/A

Kidney

UPM314

BLD

Shelter

Platynosomum sp.

Kidney

UPM315

BLD

Shelter

Platynosomum sp.

Kidney

UPM316

BLD

Shelter

Platynosomum sp.

Kidney

UPM317

BLD

Shelter

N/A

Kidney

UPM318

BLD

Shelter

N/A

Kidney

UPM320

BLD

Shelter

N/A

Kidney

UPM321

3.89 ± 0.08

Shelter

N/A

  1. BLD denotes below limit of detection of qRT-PCR; and
  2. N/A denotes not available