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Table 2 Molecular identification and genotyping of Brucella isolates

From: New Brucella variant isolated from Croatian cattle

Method(s) Reference Result
PCR AMOS Bricker &Halling, 1993 Isolates 6, 7, 11: Brucella melitensis
Bruce ladder Lopez-Goni et al. 2008 Isolate 6: Brucella melitensis
Isolate 7: Brucella abortus
Isolate 11:Brucella melitensis
PCR omp31 Vizcaino et al., 1997 Isolate 6:positive
Isolate 7: negative
Isolate 11: positive
Suis ladder Lopez-Goni et al., 2011 Unique pattern for all B. melitensis strains
MLVA 16 (Bruce06–08 - 11 - 12 - 42 - 43- 45 - 55, 18–19 - 21, 04–07 - 09 - 16 - 30) La Fleche et al., 2006; Al Dahouk et al., 2007 Isolate 6: 1–5–3-13-3-2-3-2-4-41-8-5-4-3-7-6
Isolate 7: genotype (1–5–3-13-2-2-3-2-4-41-8-5-4-3-7-6), consistent with B. melitensis
MLST 9 (gap - aroA - glk - dnaK - gyrB - trpE - cobQ - int-hyp (orf1)-omp25) Whatmore et al., 2007 Isolates 6 and 7: sequence type 8, 3–2–3-2-1-5-3-8-2; consistent with B. melitensis
WGS/wgSNP Illumina NexteraXT guide (no. 150319425031942), following protocol revision C DDBJ/ENA/GenBank under accession numbers CVI_6 ChI CP058599, CVI_6 ChII CP058600, CVI_7 ChI CP058597, and CVi_7 ChII CP058598