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Fig. 3 | BMC Veterinary Research

Fig. 3

From: Development of an indirect ELISA for detection of anti-Mycoplasma hyopneumoniae IgG in naturally infected pathogen-induced convalescent sera

Fig. 3

Specificity and sensitivity of the M. hyopneumoniae convalescent serum IgG-ELISA. a The ELISA detected no cross reactions with sera containing antibodies against 7 porcine respiratory pathogens, including M. hyorhinis (Mhr), A. pleuropneumoniae (App), S. suis serotype 2 (SS2), classical swine fever virus (CSFV), porcine reproductive and respiratory syndrome virus (PRRSV), porcine circovirus type 2 (PCV2) and pseudorabies virus gB protein (gB-PRV). b Five sera gave positive results at dilutions of 1:100, 1:500, 1:1,000 and 1:2,000, 1 serum gave a positive result at a dilution of 1:4,000, and 5 sera gave negative results at dilutions of 1:8,000 or more. Convalescent serum could be diluted up to 2,000 times for this assay

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