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Fig. 2 | BMC Veterinary Research

Fig. 2

From: Development of an indirect ELISA for detection of anti-Mycoplasma hyopneumoniae IgG in naturally infected pathogen-induced convalescent sera

Fig. 2

Optimization of the working conditions of the M. hyopneumoniae convalescent serum IgG-ELISA. Optimal coating antigen concentration was 0.25 µg/mL in coating buffer (a). The optimal blocking buffer was 2.5 % skim milk dissolved in PBS (b), and the optimal incubation time for the blocking step was 1 h (c). The optimal dilution of serum and secondary antibody were 1:500 (d) and 1:10,000 (f) diluted in blocking buffer. The optimal incubation times for serum and secondary antibody were 0.5 h (e) and 1 h (g). The optimal colorimetric reaction time was 15 min (h)

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BMC Veterinary Research

ISSN: 1746-6148

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