TY - JOUR AU - Tian, Yaqin AU - Xu, Zuobo AU - Wen, Yukang AU - Yang, Mei AU - Ning, Yaru AU - Wang, Zhaodi AU - Ding, Honglei PY - 2021 DA - 2021/03/16 TI - Development of an indirect ELISA for detection of anti-Mycoplasma hyopneumoniae IgG in naturally infected pathogen-induced convalescent sera JO - BMC Veterinary Research SP - 123 VL - 17 IS - 1 AB - Immunization of pigs with an inactivated Mycoplasma hyopneumoniae vaccine (bacterin) generates hyperimmune serum that contains high concentrations of anti-M. hyopneumoniae IgG. Commercially available IgG-ELISA kits cannot distinguish between anti-M. hyopneumoniae IgG in inactivated bacterin-induced hyperimmune sera and convalescent sera resulting from natural M. hyopneumoniae infection. Establishment of an ELISA to detect anti-M. hyopneumoniae IgG in convalescent sera will facilitate the evaluation of the M. hyopneumoniae status of pig farms. SN - 1746-6148 UR - https://doi.org/10.1186/s12917-021-02828-7 DO - 10.1186/s12917-021-02828-7 ID - Tian2021 ER -