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Table 1 PCR conditions and primers used in the PCR protocols for detecting Anaplasma/Ehrlichia spp., Borrelia burgdorferi sensu lato and Babesia/Theileria spp

From: Granulocytic anaplasmosis in captive ring-tailed lemur (Lemur catta) in Poland

Pathogen Primers Gene target Amplicon size PCR condition Reference
Anaplasma/Ehrlichia spp. EHR 521: (5′-TGT AGG CGG TTC GGT AAG TTA AAG-3′)
EHR 747: (5′-GCA CTC ATC GTT TAC AGC GTG-3′)
16 S 247 bp 35 cycles: denaturation stage at 94 °C for 30 s, annealing at 56 °C for 30 s,
elongation at 72 °C for 45 s
[14]
Borrelia burgdorferi s. l. SC1: (5′-GCT GTC AGT GCG TCT TAA-3′)
SC2: (5′-CTT AGC TGC TGC CTC CGT A-3′)
16 S 300 bp 35 cycles: denaturation stage at 94 °C for 60 s, annealing at
47 °C for 30 s, elongation at 72 °C for 90 s
[12]
Babesia/Theileria spp. RLB R2: (5’-CTA AGA ATT TCA CCT CTG ACAGT-3’)
RLB F2 (5’- GAC ACA GGG AGG TAG TGA CAAG-3’)
hypervariable V4 region of the 18 S rRNA 390–430 bp 40 cycles: denaturation stage at 94 °C for 35 s, annealing at 51 °C for 35 s,
elongation at 72 °C for 35 s
[13]