Table 1 PCR conditions and primers used in the PCR protocols for detecting Anaplasma/Ehrlichia spp., Borrelia burgdorferi sensu lato and Babesia/Theileria spp
From: Granulocytic anaplasmosis in captive ring-tailed lemur (Lemur catta) in Poland
Pathogen | Primers | Gene target | Amplicon size | PCR condition | Reference |
|---|---|---|---|---|---|
Anaplasma/Ehrlichia spp. | EHR 521: (5′-TGT AGG CGG TTC GGT AAG TTA AAG-3′) EHR 747: (5′-GCA CTC ATC GTT TAC AGC GTG-3′) | 16 S | 247 bp | 35 cycles: denaturation stage at 94 °C for 30 s, annealing at 56 °C for 30 s, elongation at 72 °C for 45 s | [14] |
Borrelia burgdorferi s. l. | SC1: (5′-GCT GTC AGT GCG TCT TAA-3′) SC2: (5′-CTT AGC TGC TGC CTC CGT A-3′) | 16 S | 300 bp | 35 cycles: denaturation stage at 94 °C for 60 s, annealing at 47 °C for 30 s, elongation at 72 °C for 90 s | [12] |
Babesia/Theileria spp. | RLB R2: (5’-CTA AGA ATT TCA CCT CTG ACAGT-3’) RLB F2 (5’- GAC ACA GGG AGG TAG TGA CAAG-3’) | hypervariable V4 region of the 18 S rRNA | 390–430 bp | 40 cycles: denaturation stage at 94 °C for 35 s, annealing at 51 °C for 35 s, elongation at 72 °C for 35 s | [13] |