Table 3 Primers used in this study
Gene | Primer | Sequence (5’→3’) | Amplicon (bp) | Reference |
|---|---|---|---|---|
Tick rrs | 16SF | F: GTATTTTGACTATACAAAGGTATTG | 300 | [55] |
16SR | R: TATTACGCTGTTATCCCTAGAGTATT | |||
gltA | Ric-CS409d | F: CCTATGGCTATTATGCTTGC | 720 | [57] |
Ric-CS535d | F: GCAATGTCTTATAAATATTC | |||
Ric-CS1258n | R: ATTGCAAAAAGTACAGTGAACA | |||
Ric-CS2d | F: ATGACCAATGAAAATAATAAT | 1200 | [7] | |
Ric-CSEndr | R: CTTATACTCTCTATGTACA | |||
17-kDa | Rr17k.1p | F: TTTACAAAATTCTAAAAACCAT | 450 | [56] |
Rr17k.90p | F: GCTCTTGCAACTTCTATGTT | |||
Rr17k.539n | R: TCAATTCACAACTTGCCATT | |||
Rickettsial rrs | Ric-16SF | F: GAACGAACGCTATCGGTATGC | 1300 | This study |
Ric-16SR1 | R: AATTTTACCGTGGTTGGCTGC | |||
Ric-16SR2 | R: TGCCTCTTGCGTTAGCTCAC | |||
groEL | Ric-ESL-F1 | F: GGTAAATGGGCAGGYACCGAA | 1500 | This study |
Ric-ESL-R1 | R: GAAGCAACRGAAGCAGCATCTTG | |||
Ric-ESL-F2 | F: ATCGTTATGAAAGAAAGCGAYG | 1500 | This study | |
Ric-ESL-R2 | R: AGWGCAGTACGCACTACTTTAGC | |||
ompA | Rr190k.71p | F: TGGCGAATATTTCTCCAAAA | 530 | [56] |
Rr190k.602n | R: AGTGCAGCATTCGCTCCCCCT | |||
Rr190k.720n | R: TGCATTTGTATTACCTATTGT |