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Table 4 Intra-assay of indirect ELISA

From: Establishment and evaluation of an indirect ELISA for detection of antibodies to goat Klebsiella pneumonia

Serum number ELISA plate batch (OD450 values) Average (X) Standard deviation (SD) Coefficient of variation (CV%)
A1 A2 A3 A4 A5 A6
P1 1.196 1.249 1.361 1.296 1.362 1.321 1.298 0.0597 4.60
P2 1.122 1.227 1.241 1.254 1.177 1.255 1.213 0.0483 3.98
P3 1.205 1.202 1.281 1.216 1.259 1.164 1.221 0.0386 3.16
P4 1.099 1.141 1.124 1.079 1.113 1.101 1.110 0.0197 1.78
N1 0.177 0.186 0.16 0.164 0.184 0.174 0.174 0.0096 5.49
N2 0.189 0.19 0.175 0.154 0.183 0.191 0.180 0.0130 7.20
N3 0.192 0.199 0.174 0.195 0.182 0.186 0.188 0.0084 4.46
N4 0.183 0.168 0.174 0.188 0.174 0.188 0.179 0.0076 4.26
  1. Note: P1-P4 are positive serum samples for Klebsiella pneumoniae, N1-N4 are negative serum samples, and A1-A6 are samples from the same batch