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Table 3 Indirect ELISA sensitivity measurement

From: Establishment and evaluation of an indirect ELISA for detection of antibodies to goat Klebsiella pneumonia

 

OD450 values of different dilutions of the Klebsiella pneumoniae positive and negative serum

Bacterial dilution

1:100

1:200

1:400

1:800

1:1600

1:800

0.892 (+)

0.758 (+)

0.324 (+)

0.293 (+)

0.241 (−)

1:800

0.162 (−)

0.163 (−)

0.152 (−)

0.126 (−)

0.146 (−)

  1. Note: (+) means positive serum, (−) means negative serum
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BMC Veterinary Research

ISSN: 1746-6148

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