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Fig. 2 | BMC Veterinary Research

Fig. 2

From: Establishment and evaluation of an indirect ELISA for detection of antibodies to goat Klebsiella pneumonia

Fig. 2

Optimization of ELISA indirect conditions. The optimal dilution of the HRP-conjugated Affinipure Rabbit Anti-Goat IgG was selected according to the P/N value (a), the antibody buffer (b), coating-buffer (c), coating-time /temperature (d), blocking-buffer (e), blocking-time/temperature (f), incubation time of the polyclonal antibody (g) and the HRP-conjugated IgG (h), and chromogenic time (i) were respectively determined in turn

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