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Table 1 Assessment of benzimidazole resistance at the dairy goat farms in the Czech Republic using the egg hatch test

From: Resistance of strongylid nematodes to anthelmintic drugs and driving factors at Czech goat farms

Farm ED50 ± SD ED99 ± SD Egg hatching ± SD (%) a Resistance status
(μg.ml-1) (μg.ml-1) 0.1 μg.ml-1 0.3 μg.ml-1
1 0.35 ± 0.05 11.72 ± 2.09 52.9 ± 5.4 37.0 ± 6.1 R
2 0.59 ± 0.07 5.69 ± 0.49 91.8 ± 1.2 73.5 ± 8.9 R
3 0.24 ± 0.03 2.19 ± 0.64 35.3 ± 1.5 21.1 ± 4.1 R
4 0.37 ± 0.07 14.19 ± 1.57 78.5 ± 1.5 53.2 ± 4.9 R
5 0.35 ± 0.05 1.00 ± 0.37 62.6 ± 13.0 48.8 ± 2.6 R
6 0.01 ± 0.01 0.36 ± 0.04 0.3 ± 0.2 0.0 ± 0.0 S
7 0.01 ± 0.02 0.03 ± 0.07 0.8 ± 0.3 0.0 ± 0.0 S
8 0.26 ± 0.03 0.56 ± 0.04 80.3 ± 6.9 24.4 ± 0.0 R
9 0.30 ± 0.01 19.24 ± 3.66 29.7 ± 6.6 28.1 ± 4.3 R
10 0.28 ± 0.04 2.07 ± 0.60 72.2 ± 6.7 42.0 ± 8.9 R
11 0.30 ± 0.03 2.15 ± 0.58 56.4 ± 3.8 28.8 ± 0.4 R
12 0.28 ± 0.03 6.79 ± 0.56 68.9 ± 3.6 40.3 ± 4.1 R
13 0.25 ± 0.05 1.68 ± 0.11 62.6 ± 6.5 32.5 ± 2.9 R
14 0.36 ± 0.06 17.52 ± 3.61 69.7 ± 7.5 53.5 ± 6.2 R
15 0.01 ± 0.01 0.03 ± 0.01 5.0 ± 0.8 2.1 ± 1.7 S
16 0.39 ± 0.08 0.94 ± 0.07 51.2 ± 4.0 43.4 ± 10.1 R
17 0.62 ± 0.05 14.10 ± 1.15 81.4 ± 3.6 69.1 ± 1.7 R
18 0.39 ± 0.06 1.02 ± 0.17 79.2 ± 3.2 48.6 ± 9.3 R
19 0.01 ± 0.01 0.04 ± 0.01 12.1 ± 3.4 7.1 ± 4.8 S
20 0.37 ± 0.07 5.19 ± 0.94 43.3 ± 0.7 27.8 ± 5.5 R
21 0.25 ± 0.05 2.70 ± 0.59 55.4 ± 4.3 23.6 ± 2.8 R
22 0.01 ± 0.00 0.57 ± 0.18 0.4 ± 0.1 0.0 ± 0.0 S
23 0.23 ± 0.04 15.86 ± 1.63 56.2 ± 5.4 45.7 ± 2.2 R
24 0.01 ± 0.03 0.03 ± 0.02 11.8 ± 2.6 7.3 ± 2.7 S
  1. Effective doses (ED50 and ED99) of thiabendazole are expressed as the mean of three measurements ± the standard deviation (SD), a percentage of nematode eggs that hatched at thiabendazole concentrations of 0.1 and 0.3 μg.ml-1, S herd susceptible to benzimidazoles, R herd resistant to benzimidazoles (ED50 > 0.2 μg/ml TBZ and > 20% of the eggs hatched at 0.3 μg.ml-1)