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Table 2 Details of the antibodies used for immunohistochemistry and immunofluorescence in canine mammary tumors

From: Characterization of neoplastic cells outlining the cystic space of invasive micropapillary carcinoma of the canine mammary gland

ANTIBODY SOURCE CLONE DILUTION MANUFACTURER ANTIGEN RETRIEVAL INCUBATION
CD31 mouse JC70A 1:100 DAKO Pressurized Heating (125°) Overnight (4°)
Cytokeratin mouse AE1/AE3 1:100 DAKO Pressurized Heating (125°) Overnight (4°)
Factor VIII- associated factor rabbit polyclonal 1:800 DAKO Pressurized Heating (125°) Overnight (4°)
Lamin B1 rabbit polyclonal 1:100 ABCAM Pressurized Heating (125°) Overnight (4°)
Lamin B2 mouse monoclonal 1:100 ABCAM Pressurized Heating (125°) Overnight (4°)
MUC1 rabbit EP1024Y 1:200 ABCAM Pressurized Heating (125°) Overnight (4°)
αSMA mouse 1A4 1:100 DAKO Pressurized Heating (125°) Overnight (4°)
S100A4 rabbit polyclonal 1:100 Thermo Scientific Pressurized Heating (125°) Overnight (4°)
p63 mouse 4A4 1:100 Neomarkers Pressurized Heating (125°) Overnight (4°)
Vimentin mouse 3B4 1:100 DAKO Pressurized Heating (125°) Overnight (4°)