Table 2 Details of the antibodies used for immunohistochemistry and immunofluorescence in canine mammary tumors
ANTIBODY | SOURCE | CLONE | DILUTION | MANUFACTURER | ANTIGEN RETRIEVAL | INCUBATION |
|---|---|---|---|---|---|---|
CD31 | mouse | JC70A | 1:100 | DAKO | Pressurized Heating (125°) | Overnight (4°) |
Cytokeratin | mouse | AE1/AE3 | 1:100 | DAKO | Pressurized Heating (125°) | Overnight (4°) |
Factor VIII- associated factor | rabbit | polyclonal | 1:800 | DAKO | Pressurized Heating (125°) | Overnight (4°) |
Lamin B1 | rabbit | polyclonal | 1:100 | ABCAM | Pressurized Heating (125°) | Overnight (4°) |
Lamin B2 | mouse | monoclonal | 1:100 | ABCAM | Pressurized Heating (125°) | Overnight (4°) |
MUC1 | rabbit | EP1024Y | 1:200 | ABCAM | Pressurized Heating (125°) | Overnight (4°) |
αSMA | mouse | 1A4 | 1:100 | DAKO | Pressurized Heating (125°) | Overnight (4°) |
S100A4 | rabbit | polyclonal | 1:100 | Thermo Scientific | Pressurized Heating (125°) | Overnight (4°) |
p63 | mouse | 4A4 | 1:100 | Neomarkers | Pressurized Heating (125°) | Overnight (4°) |
Vimentin | mouse | 3B4 | 1:100 | DAKO | Pressurized Heating (125°) | Overnight (4°) |