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Fig. 1 | BMC Veterinary Research

Fig. 1

From: Freeze-thawing impairs the motility, plasma membrane integrity and mitochondria function of boar spermatozoa through generating excessive ROS

Fig. 1

The ROS level of frozen-thawed spermatozoa with or withou NAC was detected by flow cytometry using DCFH-DA assay (a, b). The percentage of sperm motility with or withou NAC at each step of cryopreservation was determined using a computer-assisted semen analysis system (c). Data are presented as the means ± SE (n = 3). * P < 0.05, ** P < 0.01 VS the extension period of control group; # P < 0.05, ## P < 0.01 VS the thawed 30 min or thawed 240 min period of NAC group

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