Table 1 Comparison of cell densities between Cell Factory and CelCradle™ -500A culture systems
Vero cells | LT cells | |||
|---|---|---|---|---|
CelCradle™-500A | Cell Factory | CelCradle™-500A | Cell Factory | |
Inoculum (cell/ cm2) | 10,000 | 40,000 | 15,000 | 40,000 |
Harvest (cell/ cm2) | 192,000 | 150,000 | 43,000 | 98,000 |
Incubation time (days) | 5 | 4 | 8 | 6 |
Fold-increase in cell numbers | × 19 | × 3.7 | × 3 | × 2.5 |
Cell doubling time (h) | 28 h | 24 h | 86 h | 76 h |
Medium volume (ml/cm2) | 32 ml | 237 ml | 32 ml | 237 ml |