Fig. 7

A map for the putative binding sites of transcription factors on the promoter of TNFA and IL1B and relative binding activity of NF-κB on the promoter of TNFA and IL1B. a Numbers refer to the position relative to the transcriptional starting site, indicated by black arrows. The position of the transcription factors is indicated by the respective symbols. The positions of primers used for chromatin immunoprecipitation assays are denoted by dark lines surround the symbol of NF-κB. The identification for target promoter regions of candidate genes were determined by BLAST analyses as DNA-sequences that are 5′-upstream of the mRNA sequences deposited in the NCBI: NM_173966.3 (TNFA), NM_174093.1 (IL1B). b Level of NF-κB binding to the TNFA and IL1B promoter. Six individual samples in each group were involved in generating chromatin immunoprecipitation analysis. The letters in superscript indicate that the difference between groups was significant (P < 0.05)