Fig. 6

Expression of proteins related to histone H3 and acetylation of H3K14. a, b Immunoblots and acquisition of intensity from the respective blots. The protein expression was normalized by the respective abundance of GAPDH. Full-length blots are presented in Additional file 1 Fig. S1E. b, c Effect of pretreatment with metformin on the expression of acetylated histone H3 protein in pbMEC treated with LPS. Cells were exposed to LPS for 6 h with or without pretreatment with metformin for 12 h. Afterward, immunofluorescence for H3K14ac (FITC) was performed, and the nuclear was stained with dye DAPI (blue). All results are expressed as the means ± SD. Con, control; LPS, lipopolysaccharide; Metf, metformin; ML, LPS with metformin pretreatment. The letters in superscript indicate that the difference between groups was significant (P < 0.05)