Fig. 5From: Metformin activated AMPK signaling contributes to the alleviation of LPS-induced inflammatory responses in bovine mammary epithelial cellsExpression of proteins related to pro-inflammation, inflammasome and cytokines. a, b Expression of pro-inflammatory proteins. c, d Expression of proteins related to inflammasome. Immunoblots and acquisition of intensity from the respective blots. The protein expression was normalized by the respective abundance of GAPDH. Full-length blots are presented in Additional file 1 Fig. S1C and S1D. e Cytokines secreted into cultured medium by ELISA analysis. All results are expressed as the means ± SD. Con, control; LPS, lipopolysaccharide; Metf, metformin; ML, LPS with metformin pretreatment. The letters in superscript indicate that the difference between groups was significant (P < 0.05)Back to article page