Table 2 Primer sequences used for qRT-PCR
Gene | Â | Sequence | Amplicon (bp) |
|---|---|---|---|
SCX | S | 5′ GAC CGC ACC AAC AGT GTG AA | 231 |
A | 5′ TGG TTG CTG AGG CAG AAG GT | ||
COL1A1 | S | 5′ GAA AAC ATC CCA GCC AAG AA | 231 |
A | 5′ GAT TGC CAG TCT CCT CAT CC | ||
COL3A1 | S | 5′ AGG GGA CCT GGT TAC TGC TT | 215 |
A | 5′ TCT CTG GGT TGG GAC AGT CT | ||
COMP | S | 5′ TCA TGT GGA AGC AGA TGG AG | 223 |
A | 5′ TAG GAA CCA GCG GTA GGA TG | ||
RUNX2 | S | 5′ CAG ACC AGC AGC ACT CCA TA | 177 |
A | 5′ CAG CGT CAA CAC CAT CAT TC | ||
SPARC | S | 5′ AAC CTT CTG ACC GAG AAG CA | 190 |
A | 5′ TGG GAC AGG TAC CCA TCA AT | ||
ALP | S | 5′ TGG GGT GAA GGC TAA TGA GG | 221 |
A | 5′ GGC ATC TCG TTG TCC GAG TA | ||
SOX-9 | S | 5′ GAA CGC ACA TCA AGA CGG AG | 304 |
A | 5′ CTG GTG GTC TGT GTA GTC GT | ||
COL2A1 | S | 5′ AGC AGG AAT TTG GTG TGG AC | 223 |
A | 5′ TCT GCC CAG TTC AGG TCT CT | ||
ACAN | S | 5′ GAC GCC GAG AGC AGG TGT | 202 |
A | 5′ AAG AAG TTG TCG GGC TGG TT | ||
COL10A1 | S | 5′ TGC CAA CCA GGG TGT AAC AG | 244 |
A | 5′ ACA TTA CTG GGG TGC CGT TC | ||
EF1α | S | 5′ CCC GGA CAC AGA GAC TTC AT | 328 |
A | 5′ AGC ATG TTG TCA CCA TTC CA |