Fig. 5

Osteogenesis of fTDC and tTDC. a Alizarin Red staining of day 0 and 21 osteogenic cultures (Figure panels include three independent experiments). Scale bar = 100 microns. b Alkaline phosphatase bioactivities of day 0 and 21 fTDC and tTDC osteogenic cultures normalized to DNA content. c Relative osteogenic gene expression. Box-whisker plots represent medians and inter-quartile ranges. * represents significant difference (P ≤ 0.05) from respective terminally differentiated cells. ✢ represents significant difference (P ≤ 0.05) between day 21 fTDC and tTDC. n = 5