G1-like M and PB2 genes are preferentially incorporated into H7N9 progeny virions during genetic reassortment

BMC Veterinary Research

Table 2 Primers and MGB probes used in qPCR in this study and targeting genes they used to detect

Primer /probe	Sequence(5′ to 3′)	Gene detected
M-F₉₄₈	TGCCTGAGTCTATGAGGGAAG	GD15-M, SH14-M, CZ73-M, AH320-M
M-R₁₀₁₂	ACCATCGTCAACATCCACAG	GD15-M, SH14-M, CZ73-M, AH320-M
SH14-M_probe974	FAM-CGGCAGGAGCAACAGAG-MGB	SH14-M
GD15-M_probe974	VIC-CAGCATTCTGCCGTTCCT-MGB	GD15-M
M_{probe975–17}	VIC-AGCATTCTGCTGTTCCT-MGB	CZ73-M, AH320-M
PB2-F₆₅₀	TTGCTCCTTTAATGGTGGC	GD15-PB2, SH14-PB2, CZ73-PB2, AH320-PB2
PB2-R₇₇₂	AGGTCCCTTGAGTCAAATGC	GD15-PB2, SH14-PB2, CZ73-PB2, AH320-PB2
SH14-PB2_probe713	FAM-CGGTAGCAGGTGGAACAA-MGB	SH14-PB2
GD15-PB2_probe713	VIC-CAGTGGCTGGAGGGACA-MGB	GD15-PB2
PB2_probe974RC	VIC-TTGTCCCTCCAGCTACTGG-MGB	CZ73-PB2, AH320-PB2

ISSN: 1746-6148