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Fig. 4 | BMC Veterinary Research

Fig. 4

From: G1-like M and PB2 genes are preferentially incorporated into H7N9 progeny virions during genetic reassortment

Fig. 4

Schematic representation of reassortant viruses and their growth kinetics. (A) Reassortant virus generated from eight plasmid of GD15 virus. (B) Reassortant virus bearing the genetic backbone of GD15 virus, with the g1GD15-M gene being replaced by f98SH14-M gene. (C) Reassortant virus bearing the genetic backbone of GD15 virus, with the g1GD15-PB2 being replaced by f98SH14-PB2 gene. (D) Reassortant virus bearing the genetic backbone of GD15 virus, with g1GD15-M and g1GD15-PB2 being replaced by with f98SH14-M and f98SH14-PB2 genes. (E) The growth Kinetics of the reassortant viruses in MDCK cells. MDCK cells were infected with virus at an MOI of 0.01. At the indicated times after infection, virus titeres in the supernatant were determined. Data represent the mean ± SD (n = 3) from triplicate independent infections. The statistically significant differences were analyzed by ANOVA compared with WT-GD15 virus (*P < 0.05; **P < 0.01; ***P < 0.001)

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