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Fig. 3 | BMC Veterinary Research

Fig. 3

From: G1-like M and PB2 genes are preferentially incorporated into H7N9 progeny virions during genetic reassortment

Fig. 3

Schematic representation of the system used to perform cotransfection and coinfection. (A & B) Competitive between g1CZ73-M/PB2 and f98SH14-M/PB2 in backgroud of GD15 with PB2 and M from CZ73 and M/PB2 from SH14 during cotransfection. (E & F) Competitive between g1CZ73-M/PB2 and f98SH14-M/PB2 during cotransfection, where the internal genes of GD15 were replaced by those of CZ73. (I & K) Competitive between g1AH320-M/PB2 and f98SH14-M/PB2 in backgroud of GD15 with PB2 and M from AH320 and M/PB2 from SH14 during cotransfection. 500 ng of each plasmid was used in above cotransfections. (C & D & G & H & J & L) Quantitative analysis of PB2 and M genes in allantoic fluid. Data are represented as mean ± SD (N = 6). The statistically significant differences were analyzed by ANOVA (*P < 0.05; **P < 0.01; ***P < 0.001)

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BMC Veterinary Research

ISSN: 1746-6148

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