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Fig. 2 | BMC Veterinary Research

Fig. 2

From: G1-like M and PB2 genes are preferentially incorporated into H7N9 progeny virions during genetic reassortment

Fig. 2

Competitive between g1GD15-M/PB2 and f98SH14-M/PB2 genes during cotransfection. (A & D) Schematic representation of the plasmids, and different amount of M and PB2 plasmids were used in the cotransfections. (B&C) Quantitative analysis of g1GD15-M and f98SH14-M genes in virus population expanded from the contransfection. 293 T cells were cotransfected with nine plasmids, supernatants were collected and inoculated into eggs. qRT-PCR was performed to determine the numbers of g1GD15-M and f98SH14-M genes. (E & F) Quantitative analysis of g1GD15-PB2 and f98SH14-PB2 genes in virus population expanded from the contransfection. 293 T cells were cotransfected with nine plasmids, 500 ng (E) and 250 ng (F) of g1GD15-PB2 plasmid was used respectively. Supernatants were collected and inoculated into eggs. qRT-PCR was performed to determine the numbers of g1GD15-M and f98SH14-M genes in allantoic fluid. Data are represented as mean ± SD (N = 8). The statistically significant differences were analyzed by ANOVA (*P < 0.05; **P < 0.01; ***P < 0.001)

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