Fig. 3

Effects of PRRSV isolate, TVclass, and WUR on gene expression in tonsil based on the NanoString data when accounting for cell enrichment, as predicted by Ingenuity Pathway analysis. a The three significant biological functions in orange were predicted to be more activated, or less inhibited, in tonsils of KS06-infected pigs compared to NVSL-infected pigs (magnitude indicated by intensity of red or green). b Regulators in blue were predicted to indirectly regulate (dashed line) their downstream differentially expressed genes for TVclass High/Low in red (expressed more in High TVclass) or green (expressed less in High TVclass), which were predicted to have effects on activating “formation of granuloma” or increasing “Quantity of IFNG in blood” (in orange) in tonsils of High-TVclass pigs compared to Low-TVclass pigs. c Differentially expressed genes for WUR genotype have effects on five biological functions (orange/blue represents activation/inhibition of the functions). The genes in red/green were expressed more/less in AB pigs than in AA pigs (magnitude indicated by intensity of red/green). The dashed lines represent indirect interactions; the blunt arrow indicates negative regulation and “➔” indicates positive regulation. The synonym of “Antibody-dependent cell-mediated cytotoxicity” is regulation of natural killer cell mediated cytotoxicity