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Fig. 6 | BMC Veterinary Research

Fig. 6

From: Immunogenicity study of a Streptococcus suis autogenous vaccine in preparturient sows and evaluation of passive maternal immunity in piglets

Fig. 6

Experiment 2: Kinetics of total Ig against S. suis serotype 7 in piglets from either vaccinated or non-vaccinated gilts. a Randomly selected 145 piglets (5 piglets/gilt) from vaccinated and non-vaccinated gilts were sampled at 7 and 18 days of age and total Ig [IgG + IgM] titers were determined by ELISA against S. suis serotype 7. Individual antibody titers are shown with horizontal bars representing mean ± SEM. Values significantly different are shown in the graph with corresponding P value. b Blood samples from randomly selected 25 piglets from vaccinated and from non-vaccinated gilts (sampled at 7 days of age) were used to evaluate antibody functionality in an opsonophagocytosis assay (OPA). For OPA, blood leukocytes were mixed with S. suis serotype 7 (vaccine strain) at a multiplicity of infection of 0.01. Control sera or sample sera were added to a final concentration of 40% v/v in microtubes which were incubated for 4 h. After incubation, viable bacterial counts were performed, and the percentage of bacterial killing determined. Results are expressed as the % of S. suis killing for individual serum, with horizontal bars representing mean ± SEM

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