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Fig. 4 | BMC Veterinary Research

Fig. 4

From: Immunogenicity study of a Streptococcus suis autogenous vaccine in preparturient sows and evaluation of passive maternal immunity in piglets

Fig. 4

Experiment 1: Opsonophagocytosis killing of S. suis serotype 7 induced by serum antibodies from vaccinated sows and from their piglets. Blood samples were collected 1 week before farrowing from 25 vaccinated sows and from randomly chosen piglets (n = 50; 2 per sow) at 7 and 18 days of age to evaluate antibody functionality in an opsonophagocytosis assay (OPA). For OPA, blood leukocytes were mixed with S. suis serotype 7 (vaccine strain) at a multiplicity of infection of 0.01. Control sera or sample sera were added to a final concentration of 40% v/v in microtubes which were incubated for 4 h. After incubation, viable bacterial counts were performed, and the percentage of bacterial killing determined. Results are expressed as the % of S. suis killing for individual serum, with horizontal bars representing mean ± SEM. Values significantly different are shown in the graph with corresponding P value

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