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Table 3 The effect of the inclusion of safflower oil and vitamin C in the broiler chicken diet on the intestine’s morphometric measures

From: Changes in the growth, ileal digestibility, intestinal histology, behavior, fatty acid composition of the breast muscles, and blood biochemical parameters of broiler chickens by dietary inclusion of safflower oil and vitamin C

Item

Vitamin C level (mg/kg diet)

Safflower oil level (gm/kg diet)

Vitamin C × Safflower oil

SEM

P-value

0

400

0

5

10

0Vit.C+ 0Saff. Oil

0Vit.C + 5 Saff. Oil

0Vit.C+ 10 Saff. Oil

400Vit.C+

0Saff. Oil

400Vit.C+

5 Saff. Oil

400Vit.C+

10Saff. Oil

 

Vit. C

Saff. oil

Vit. C x Saff. oil

Duodenum

 Villous height

1062.06

1153.6

777.17b

1314.63a

1231.77a

749.15b

1461.17a

975.87b

889.01b

1149.3ab

1487.6a

80.75

0.52

<  0.01

<  0.01

 Villous width

176.40

134.42

85.84

181.84

198.56

15.09b

260.98a

253.14a

140.30ab

111.89ab

143.97ab

26.19

0.36

0.08

<  0.01

 Crypt depth

286.02

241.77

98.61b

482.31a

210.76b

33.64d

616.55a

207.86c

189.42cd

366.49b

213.66bc

45.77

0.58

0.00

<  0.01

 VH/CD

10.12

5.09

13.90a

2.94b

5.97b

22.91a

2.44c

5.01ab

4.89ab

3.44ab

6.93b

1.72

0.09

<  0.01

<  0.01

 Mucosal depth

152.98b

182.71a

92.55b

236.44a

174.56ab

21.52e

301.97a

135.46d

163.34cd

173.75c

213.66b

20.48

0.41

<  0.01

<  0.01

 GCC

121.42a

15.33b

20.13b

25.13b

159.88a

11.75b

140.75b

311.75a

25.60b

8.00b

8.00b

27.22

0.02

0.01

<  0.01

 IELI

46.75a

22.00b

15.25

49.25

38.63

9.00b

77.50a

53.75ab

19.20b

24.67b

23.50ab

7.59

0.05

0.09

<  0.01

Jejunum

 Villous height

880.92

865.20

895.05

843.87

880.27

840.36

797.62

1004.79

937.34

890.90

755.76

38.96

0.82

0.82

0.28

 Villous width

167.13

128.81

57.75b

214.22a

171.94a

10.16c

282.80a

208.44ab

103.01bc

162.95b

135.45b

22.71

0.34

<  0.01

<  0.01

 Crypt depth

198.27

214.03

117.52b

259.83a

241.09a

23.71b

311.45a

259.65a

197.82a

246.38a

222.53a

26.00

0.73

0.01

<  0.01

 VH/CD

14.98a

4.29b

20.95a

3.81b

4.15b

37.46a

2.58b

4.89b

4.43b

5.05b

3.40b

3.13

0.04

<  0.01

<  0.01

 Mucosal depth

163.05b

189.99a

115.49

207.62

206.45

19.63d

279.15a

190.37bc

187.82ab

150.21c

202.53ab

20.89

0.46

0.05

<  0.01

 GCC

220.25a

11.08b

107.38

115.75

123.88

204.50a

221.25a

235.00a

9.00b

12.33b

12.75b

29.32

0.00

0.96

<  0.01

 IELI

82.75a

22.33b

23.75

65.63

68.25

17.00b

109.00a

122.25a

32.40b

16.33b

14.25b

11.40

0.001

0.11

<  0.01

Ileum

 Villous height

629.71

691.81

500.30c

651.94b

830.04a

436.86c

625.73abc

826.55a

578.01bc

692.5ab

833.52a

39.06

0.37

0.00

<  0.01

 Villous width

187.66

176.16

111.90b

207.60ab

226.22a

20.44c

226.46ab

316.08a

188.99b

207.83b

136.37b

23.50

0.78

0.04

<  0.01

 Crypt depth

110.09b

179.74a

94.61b

166.83a

173.30a

33.82c

127.34b

169.10b

175.32ab

190.10a

177.5ab

14.40

<  0.01

0.01

<  0.01

 VH/CD

7.68a

4.02b

8.38a

4.32b

4.84b

12.95a

5.19b

4.89b

3.82b

3.44b

4.80b

0.86

0.01

0.04

<  0.01

 Mucosal depth

152.91

159.03

94.55b

179.00a

194.37a

33.69b

213.80a

211.25a

149.55a

150.21a

177.50a

16.89

0.84

<  0.01

<  0.01

 GCC

219.33

158.92

127.00b

336.50a

103.88b

245.50ab

375.25a

37.25bc

87.00c

263.33a

170.5abc

38.18

0.37

<  0.01

0.01

 IELI

79.58

253.58

363.25

93.63

42.88

7.25b

160.00b

71.50b

578.20a

31.67b

14.25b

50.15

0.16

0.20

0.01

  1. a, b, c Means within the same row carrying different superscripts are significantly different (P <  0.05). VH/CD villous height/crypt depth ratio, GCC Goblet cells count, IELI Intra-epithelium lymphocytic lick cell infiltrations