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Table 4 Comparison study between the cELISA immunoassay and HI assay for the detection of H7 antibody in chicken, duck and peacock field serum samples

From: Development and evaluation of a monoclonal antibody-based competitive ELISA for the detection of antibodies against H7 avian influenza virus

Target

Chicken serum

Duck serum

Peacock serum

H7 cELISA

HI test

H7 cELISA

HI test

H7 cELISA

HI test

Positive

40

40

103

105

99

100

Negative

75

75

42

40

41

40

Positive rates

34.8%

34.8%

71.0%

72.4%

70.7%

71.4%

Sensitivity a(TP/(TP + FN))

100%

98.1%

99%

Specificity b(TN/(TN + FP))

100%

100%

100%

Accuracy c((TP + TN)/Total)

100%

98.6%

99.3%

  1. a TP true positive, FN false negative; sensitivity = (TP/(TP + FN)) × 100%
  2. b TN true negative, FP false positive; specificity = (TN/(TN+ FP)) × 100%
  3. c Total, total number of serum samples; Accuracy = ((TP + TN)/Total) × 100%
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BMC Veterinary Research

ISSN: 1746-6148

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