Development and evaluation of a monoclonal antibody-based competitive ELISA for the detection of antibodies against H7 avian influenza virus

Table 3 Intra and Inter-assay repeatability of the cELISA immunoassay

Species	Serum samples	HI titer	Inhibition percentage
			Intra-assay			Inter-assay
			Mean (%)	SD	CV (%)	Mean (%)	SD	CV (%)
Chicken	Positive serum 1	2⁶	77.30	0.014	1.92	76.34	0.052	6.91
	Positive serum 2	2⁸	78.34	0.003	0.35	70.07	0.095	13.62
	Positive serum 3	2⁹	81.50	0.005	0.67	85.76	0.049	5.74
	Negative serum 1	–	29.01	0.002	0.82	29.76	0.013	4.43
	Negative serum 2	–	14.89	0.009	6.17	19.50	0.027	14.04
Duck	Positive serum 1	2¹⁰	89.69	0.011	1.22	84.20	0.064	7.56
	Positive serum 2	2¹⁰	88.75	0.007	0.81	81.50	0.091	11.12
	Positive serum 3	2¹⁰	86.72	0.017	1.91	81.73	0.059	7.18
	Negative serum 1	–	13.00	0.002	1.57	13.21	0.004	2.94
	Negative serum 2	–	22.39	0.007	3.27	20.61	0.031	15.15
Peacock	Positive serum 1	2⁹	85.46	0.014	1.69	80.66	0.056	6.96
	Positive serum 2	2⁹	85.07	0.024	2.85	89.63	0.054	6.07
	Positive serum 3	2⁹	93.99	0.002	0.19	90.13	0.045	4.95
	Negative serum 1	–	15.94	0.001	0.20	15.66	0.014	8.74
	Negative serum 2	–	11.12	0.002	1.66	11.87	0.013	11.04

ISSN: 1746-6148