Skip to main content
Fig. 3 | BMC Veterinary Research

Fig. 3

From: Peptide enzyme‐linked immunosorbent assay (pELISA) as a possible alternative to the neutralization test for evaluating the immune response to IBV vaccine

Fig. 3

Reactivity of the peptide with immune sera against different genotypes of IBV strains. (A) Reaction with immune sera against different IBV strains. The ELISA titer means the maximum dilution of the serum sample with OD value greater than the pELISA’s cut-off. ** p<0.01; ns (nonsignificant); p >0.05. (B) These sera were proven to be positive against IBV by an IFA. a, represents SPF chicken serum; b, c, d, e, and f represent M41 immune serum (Mass-type), 4/91 serum (4/91-type), CK/CH/2014/QL1403 serum (TC07-2-type), CK/CH/2014/FJ14 serum (QX-type), and CK/CH/2010/JT1 serum (New cluster-type), respectively. (C) Comparative amino acid sequence analysis of the peptides used for pELISA and the vaccine and reference strains used for immune serum production. “+” represented the positive serum against corresponding to strain could react with the peptide

Back to article page