Fig. 2
Phagocytosis of S. aureus by macrophages over time. a, b Representative flow cytometry histograms of GFP expression in macrophages 6 h post-infection with an initial bacterial inoculum of 104 (A) or 107 (B) CFU/mL without cephalexin. The GFP- and GFP+ populations are separated. c Phagocytosis index Iphag over time following exposure of different initial inoculum sizes of S. aureus without cephalexin. d, e, f Phagocytosis index Iphag over time following exposure of an initial bacterial inoculum of 105 (d), 106 (e) or 107 (f) CFU/mL to macrophages and to different concentrations of cephalexin. Cephalexin concentrations are expressed as multiples of the MIC (cephalexin MIC = 8 μg/mL). Each symbol represents the mean of results from three experiments. Curves for MOI 0.1, 1 and 10 from C are the same ones than curves for 0 MIC from D, E and F