Identification of bacterial pathogens in cultured fish with a custom peptide database constructed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS)

Table 2 Comparison of the test results for bacterial species identification using a custom MSP database and Bruker database

Pathogen	MSP database	No. of isolate matched with 16 s rDNA sequencing (%)	Mean log score ± SD (Min - Max)
S. agalactiae	Custom	15/15 (100%)	2.599 ± 0.26 (2.244–2.999)
S. agalactiae	Bruker	9/15 (60%)	1.758 ± 0.15 (2.545–2.019)
S. iniae	Custom	15/15 (100%)	2.554 ± 0.25 (2.118–2.999)
S. iniae	Bruker	0/15 (0%)	1.753 ± 0.17 (1.432–1.997)
A. hydrophila	Custom	15/15 (100%)	2.568 ± 0.22 (2.009–2.991)
A. hydrophila	Bruker	6/15 (40%)	1.883 ± 0.20 (1.454–2.181)
A. veronii	Custom	15/15 (100%)	2.562 ± 0.25 (2.198–3.000)
A. veronii	Bruker	7/15 (46.67%)	1.808 ± 0.14 (1.559–2.027)
E. tarda	Custom	15/15 (100%)	2.554 ± 0.24 (2.116–3.000)
E. tarda	Bruker	10/15 (66.67%)	1.877 ± 0.18 (1.584–2.254)
Total	Custom	75/75 (100%)	2.568 ± 0.25 (2.009–3.000)
Total	Bruker	32/75 (42.67%)	1.816 ± 0.17 (1.432–2.254)

ISSN: 1746-6148