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Table 1 Method validation of the MALDI Biotyper of five bacterial pathogens showing the matching results with the 16 s rDNA sequencing, custom MSP and Bruker MSP database. The identity was calculated against NCBI database

From: Identification of bacterial pathogens in cultured fish with a custom peptide database constructed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS)

Isolate numberSource16S rDNA sequencingMALDI-TOF
Custom MSP databaseBruker MSP database
Organism best match% identityOrganism best matchLog scoreOrganism best matchLog score
Streptococcus spp
 S147-JaNile tilapiaS. agalactiae100S. agalactiae2.999S. parauberis1.877
 S183-JaRed tilapiaS. agalactiae100S. agalactiae2.897S. parauberis1.665
 S187-JaNile tilapiaS. agalactiae99S. agalactiae2.988S. agalactiae1.545
 S190-JaRed tilapiaS. agalactiae99S. agalactiae2.856S. agalactiae1.998
 SV1/1-JaNile tilapiaS. agalactiae100S. agalactiae2.945S. agalactiae2.019
 S71Red tilapiaS. agalactiae99S. agalactiae2.244S. urinalis1.666
 S96Red tilapiaS. agalactiae100S. agalactiae2.377S. agalactiae1.756
 S100Nile tilapiaS. agalactiae99S. agalactiae2.442S. agalactiae1.582
 S101Nile tilapiaS. agalactiae99S. agalactiae2.375S. parauberis1.829
 S102Nile tilapiaS. agalactiae98S. agalactiae2.544S. parauberis1.876
 S183Red tilapiaS. agalactiae100S. agalactiae2.388S. agalactiae1.716
 S184Red tilapiaS. agalactiae100S. agalactiae2.591S. agalactiae1.749
 S191Red tilapiaS. agalactiae99S. agalactiae2.347S. urinalis1.564
 S195Nile tilapiaS. agalactiae97S. agalactiae2.455S. agalactiae1.632
 S198Nile tilapiaS. agalactiae99S. agalactiae2.544S. agalactiae1.908
 NS12-JaRed tilapiaS. iniae100S. iniae2.898S. agalactiae1.432
 NS70-JaNile tilapiaS. iniae100S. iniae2.988S. agalactiae1.688
 NS74-JaBarramundiS. iniae100S. iniae2.878S. parauberis1.987
 NS76-JaBarramundiS. iniae99S. iniae2.999S. pyogenes1.555
 NS185-JaBarramundiS. iniae99S. iniae2.778S. parauberis1.654
 NS11Nile tilapiaS. iniae98S. iniae2.376S. agalactiae1.997
 NS18Nile tilapiaS. iniae100S. iniae2.419S. agalactiae1.707
 NS26Red tilapiaS. iniae99S. iniae2.534S. pyogenes1.728
 NS34BarramundiS. iniae96S. iniae2.544S. agalactiae1.886
 NS50BarramundiS. iniae97S. iniae2.308S. pyogenes1.679
 NS84BarramundiS. iniae98S. iniae2.118S. pyogenes1.679
 NS85BarramundiS. iniae98S. iniae2.445S. agalactiae1.864
 NS89Red tilapiaS. iniae99S. iniae2.342S. agalactiae1.975
 NS90Red tilapiaS. iniae100S. iniae2.221S. agalactiae1.873
 NS91Nile tilapiaS. iniae100S. iniae2.464S. parauberis1.593
Aeromonas spp
 A28-JaNile tilapiaA. hydrophila100A. hydrophila2.978A. veronii1.495
 A29-JaHybrid catfishA. hydrophila100A. hydrophila2.991A. veronii1.767
 A49-JaRed tilapiaA. hydrophila100A. hydrophila2.965A. hydrophila1.869
 A50-JaNile tilapiaA. hydrophila100A. hydrophila2.889A. ichthiosmia2.094
 A84-JaSnakehead fishA. hydrophila100A. hydrophila2.945A. hydrophila1.755
 A90Nile tilapiaA. hydrophila99A. hydrophila2.312A. veronii2.018
 A108Hybrid catfishA. hydrophila100A. hydrophila2.464A. hydrophila1.956
 A109Red tilapiaA. hydrophila100A. hydrophila2.115A. hydrophila1.848
 A110Snakehead fishA. hydrophila98A. hydrophila2.394A. hydrophila2.181
 A112Snakehead fishA. hydrophila98A. hydrophila2.601A. hydrophila2.011
 A114Nile tilapiaA. hydrophila97A. hydrophila2.451A. veronii1.995
 A115Nile tilapiaA. hydrophila97A. hydrophila2.551A. ichthiosmia2.045
 A120Hybrid catfishA. hydrophila99A. hydrophila2.009A. veronii1.454
 A126Snakehead fishA. hydrophila100A. hydrophila2.567A. veronii1.777
 A127Snakehead fishA. hydrophila99A. hydrophila2.301A. shigelloides1.985
 SB1-JaNile tilapiaA. veronii100A. veronii2.969A. hydrophila1.559
 SB2-JaNile tilapiaA. veronii99A. veronii3.000A. shigelloides1.997
 SB3-JaBarramundiA. veronii99A. veronii2.897A. veronii1.787
 SB4-JaBarramundiA. veronii100A. veronii2.888A. veronii1.945
 SB7-JaRed tilapiaA. veronii98A. veronii2.899A. shigelloides1.658
 SB5Nile tilapiaA. veronii98A. veronii2.327A. ichthiosmia2.027
 SB6Nile tilapiaA. veronii100A. veronii2.454A. hydrophila1.787
 SB8BarramundiA. veronii98A. veronii2.382A. hydrophila1.844
 SB9BarramundiA. veronii98A. veronii2.511A. veronii1.906
 SB10BarramundiA. veronii97A. veronii2.377A. veronii1.733
 SB12BarramundiA. veronii100A. veronii2.401A. veronii1.667
 SB13BarramundiA. veronii99A. veronii2.359A. hydrophila1.872
 SB14BarramundiA. veronii97A. veronii2.228A. ichthiosmia1.560
 SB15BarramundiA. veronii100A. veronii2.553A. veronii1.924
 SB17BarramundiA. veronii100A. veronii2.198A. veronii1.855
Edwardsiella tarda
 Ed10-JaHybrid catfishE. tarda100E. tarda2.899E. tarda2.000
 Ed12-JaHybrid catfishE. tarda100E. tarda2.932E. tarda1.887
 Ed14-JaNile tilapiaE. tarda99E. tarda2.984E. tarda1.666
 Ed16-JaNile tilapiaE. tarda98E. tarda3.000E. hoshinae1.669
 Ed18-JaNile tilapiaE. tarda100E. tarda2.999E. hoshinae1.842
 Ed8Hybrid catfishE. tarda99E. tarda2.382E. hoshinae1.584
 Ed9Hybrid catfishE. tarda100E. tarda2.367E. hoshinae1.624
 Ed11Hybrid catfishE. tarda98E. tarda2.203E. hoshinae1.782
 Ed17Nile tilapiaE. tarda99E. tarda2.471E. tarda1.977
 Ed20Nile tilapiaE. tarda100E. tarda2.457E. tarda1.945
 Ed23Hybrid catfishE. tarda98E. tarda2.457E. tarda2.254
 Ed25Hybrid catfishE. tarda100E. tarda2.342E. tarda1.963
 Ed27Nile tilapiaE. tarda100E. tarda2.264E. tarda2.014
 Ed30Nile tilapiaE. tarda100E. tarda2.445E. tarda2.002
 Ed31Nile tilapiaE. tarda100E. tarda2.116E. tarda1.956
  1. aIsolate used for MSP database construction
  2. log score values: < 1.700 = no reliable identification, ≥ 1.700–1.999 = probable genus-level identification, 2.000–2.229 = a secure genus-level identification and a probable species-level identification, and 2.300–3.000 = a secure species-level identification and highly probable species-level identification